1B2-1B7
1B2-1B7
规格:
货期:
编号:TS213000
品牌:Testobio
| 产品名称： | 1B2-1B7 |
|---|---|
| 商品货号： | TS213000 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against glycosphingolipid |
| Cellular Products： | immunoglobulin; monoclonal antibody; against glycosphingolipid |
| Comments： | Animals were immunized with lacto-N-nor-hexaosylceramide non-covalently adsorbed to naked Salmonella minnesota. Spleen cells were fused with NS-1 myeloma cells. The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue. The antibody agglutinates all adult human erythrocytes but not umbilical cord blood erythrocytes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium, 85%; fetal bovine serum, 15%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | WW Young |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Young WW Jr., et al. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-n-glycosyl type II chain. J. Biol. Chem. 256: 10967-10972, 1981. PubMed: 7287743 |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
1B2-1B7
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | 1B2-1B7 |
|---|---|
| 商品货号： | TS213000 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against glycosphingolipid |
| Cellular Products： | immunoglobulin; monoclonal antibody; against glycosphingolipid |
| Comments： | Animals were immunized with lacto-N-nor-hexaosylceramide non-covalently adsorbed to naked Salmonella minnesota. Spleen cells were fused with NS-1 myeloma cells. The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue. The antibody agglutinates all adult human erythrocytes but not umbilical cord blood erythrocytes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium, 85%; fetal bovine serum, 15%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | WW Young |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Young WW Jr., et al. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-n-glycosyl type II chain. J. Biol. Chem. 256: 10967-10972, 1981. PubMed: 7287743 |
