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16-1-2N
16-1-2N
规格:
货期:
编号:TS213022
品牌:Testobio
产品名称: 16-1-2N
商品货号: TS213022
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody cross-reacts with H-2Kq, H-2Kp and H-2Kr.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against H-2 K k, D k; cytotoxic
Cellular Products:
immunoglobulin; monoclonal antibody; against H-2 K k, D k; cytotoxic
Comments:
Mice were immunized spleen cells from C3H mice.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with H-2Kq, H-2Kp and H-2Kr.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype: IgG2a; kappa light chain
    Name of Depositor: DH Sachs, K Ozato
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699

    首页 > 产品中心 > 微生物培养 > 菌株 > null > 16-1-2N

    16-1-2N

    • 货号: TS213022
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: 16-1-2N
    商品货号: TS213022
    Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
    Cell Type: hybridoma: B lymphocyte
    Product Format: frozen
    Morphology: lymphoblast
    Culture Properties: suspension
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Applications:
    The antibody cross-reacts with H-2Kq, H-2Kp and H-2Kr.
    Tested and found negative for ectromelia virus (mousepox).
    Derivation:
    Spleen cells were fused with NS-1 myeloma cells.
    Genes Expressed:
    immunoglobulin; monoclonal antibody; against H-2 K k, D k; cytotoxic
    Cellular Products:
    immunoglobulin; monoclonal antibody; against H-2 K k, D k; cytotoxic
    Comments:
    Mice were immunized spleen cells from C3H mice.
    Spleen cells were fused with NS-1 myeloma cells.
    The antibody cross-reacts with H-2Kq, H-2Kp and H-2Kr.
    Tested and found negative for ectromelia virus (mousepox).
    Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Isotype: IgG2a; kappa light chain
    Name of Depositor: DH Sachs, K Ozato
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699

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