14-4-4S
14-4-4S
规格:
货期:
编号:TS213043
品牌:Testobio
| 产品名称： | 14-4-4S |
|---|---|
| 商品货号： | TS213043 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is highly cross-reactive and reacts with H-2k, H-2d, H-2p and H-2r. The specificity of the antibody corresponds to the Ia. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-E k; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-E k; cytotoxic |
| Comments： | Animals were immunized with spleen cells from C3H mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is highly cross-reactive and reacts with H-2k, H-2d, H-2p and H-2r. The specificity of the antibody corresponds to the Ia.7 serologic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | DH Sachs, K Ozato |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699 |
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周雪
13372109301
| 产品名称： | 14-4-4S |
|---|---|
| 商品货号： | TS213043 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is highly cross-reactive and reacts with H-2k, H-2d, H-2p and H-2r. The specificity of the antibody corresponds to the Ia. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-E k; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-E k; cytotoxic |
| Comments： | Animals were immunized with spleen cells from C3H mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is highly cross-reactive and reacts with H-2k, H-2d, H-2p and H-2r. The specificity of the antibody corresponds to the Ia.7 serologic specificity. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | DH Sachs, K Ozato |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ozato K, et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J. Immunol. 124: 533-540, 1980. PubMed: 7188699 |
