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14E5
14E5
规格:
货期:
编号:TS213045
品牌:Testobio
产品名称: 14E5
商品货号: TS213045
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody reacts with human vascular endothelial cells, mature peripheral blood macrophages and DHL-2 cells, but does not react with bovine endothelial cells or human B or T lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against human endothelium and macrophage
Cellular Products:
immunoglobulin; monoclonal antibody; against human endothelium and macrophage
Comments:
Animals were immunized with human umbilical vein endothelial cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human vascular endothelial cells, mature peripheral blood macrophages and DHL-2 cells, but does not react with bovine endothelial cells or human B or T lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium:
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1; kappa light chain
Name of Depositor: YA Reid
Deposited As: mouse (B cell); mouse (myeloma)
References:

Hamburger AW, et al. Isolation and characterization of monoclonal antibodies reactive with endothelial cells. Tissue Cell 17: 451-459, 1985. PubMed: 3901396

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14E5

  • 货号: TS213045
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: 14E5
商品货号: TS213045
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody reacts with human vascular endothelial cells, mature peripheral blood macrophages and DHL-2 cells, but does not react with bovine endothelial cells or human B or T lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation:
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against human endothelium and macrophage
Cellular Products:
immunoglobulin; monoclonal antibody; against human endothelium and macrophage
Comments:
Animals were immunized with human umbilical vein endothelial cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human vascular endothelial cells, mature peripheral blood macrophages and DHL-2 cells, but does not react with bovine endothelial cells or human B or T lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium:
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1; kappa light chain
Name of Depositor: YA Reid
Deposited As: mouse (B cell); mouse (myeloma)
References:

Hamburger AW, et al. Isolation and characterization of monoclonal antibodies reactive with endothelial cells. Tissue Cell 17: 451-459, 1985. PubMed: 3901396

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