| 产品名称： | Bovine adenovirus 4 |
|---|---|
| 商品货号： | TS213182 |
| Classification： | Adenoviridae, Atadenovirus, |
| Deposited As： | Bovine adenovirus type 4 |
| Agent： | Bovine adenovirus 4 |
| Strain： | THT/62 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Lungs of a calf with pneumoenteritis |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Does not share common CF antigen with human adenoviruses. No cross-reactivity with bovine adenovirus types 1-3. GenBank: AF036092.3 |
| Effect on Host： | CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies xa0CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies |
| Recommended Host： | BT (ATCC CRL-1390) Production Host: xa0BT (ATCC® CRL-1390x99) |
| Growth Conditions： | Temperature: 37.0°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C without CO2, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 6-7 days at 37°C without CO2, until 90% of the culture shows CPE. |
| Name of Depositor： | SB Mohanty |
| Chain of Custody： | Deposited as Bovine adenovirus type 4. Originally received after 6 passages in primary calf testicular cells. |
| Source： | Lungs of a calf with pneumoenteritis |
| References： | Bartha A, Aldasy P. Further two serotypes of bovine adenoviruses (serotype 4 and 5). Acta Vet. Acad. Sci. Hung. 16(1): 107-108, 1966. PubMed: 5917816 |
| Cross References： | GenBank : AF036092.3 Bovine adenovirus 4 strain THT/62, complete genome. |
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| 产品名称： | Bovine adenovirus 4 |
|---|---|
| 商品货号： | TS213182 |
| Classification： | Adenoviridae, Atadenovirus, |
| Deposited As： | Bovine adenovirus type 4 |
| Agent： | Bovine adenovirus 4 |
| Strain： | THT/62 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Lungs of a calf with pneumoenteritis |
| Storage Conditions： | xa0-70°C or colder |
| Comments： | Does not share common CF antigen with human adenoviruses. No cross-reactivity with bovine adenovirus types 1-3. GenBank: AF036092.3 |
| Effect on Host： | CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies xa0CPE, generalized enlarged cell rounding and cell clumping or clustering; Type B intranuclear inclusion bodies |
| Recommended Host： | BT (ATCC CRL-1390) Production Host: xa0BT (ATCC® CRL-1390x99) |
| Growth Conditions： | Temperature: 37.0°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C without CO2, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: Incubate infected culture for 6-7 days at 37°C without CO2, until 90% of the culture shows CPE. |
| Name of Depositor： | SB Mohanty |
| Chain of Custody： | Deposited as Bovine adenovirus type 4. Originally received after 6 passages in primary calf testicular cells. |
| Source： | Lungs of a calf with pneumoenteritis |
| References： | Bartha A, Aldasy P. Further two serotypes of bovine adenoviruses (serotype 4 and 5). Acta Vet. Acad. Sci. Hung. 16(1): 107-108, 1966. PubMed: 5917816 |
| Cross References： | GenBank : AF036092.3 Bovine adenovirus 4 strain THT/62, complete genome. |
