| 产品名称： | Hepatitis A virus |
|---|---|
| 商品货号： | TS213410 |
| Classification： | Picornaviridae, Hepatovirus |
| Deposited As： | Hepatitis A |
| Agent： | Hepatitis A virus |
| Strain： | HAS-15 |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Comments： | Genome contains 18-nucleotide deletion between VPI nucleotides 3383-2301. Attenuated for chimpanzees at 200 ng dose. |
| Effect on Host： | Gradual deterioration of cells; titer by ELISA or by RT-PCR (cells become persistently infected). Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate, but remain attached indicating persistent infection. |
| Recommended Host： | FRhK-4 (ATCC® CRL-1688™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 14-21 days at 37°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | BH Robertson |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Human feces |
| References： | Robertson BH, et al. Large scale production of hepatitis A virus in cell culture: effect of type of infection on virus yield and cell integrity. J. Gen. Virol. 69: 2129-2134, 1988. PubMed: 2841419 |
| Cross References： | Nucleotide (GenBank) : JQ425480.1 Hepatitis A virus strain HAS-15, complete genome |
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| 产品名称： | Hepatitis A virus |
|---|---|
| 商品货号： | TS213410 |
| Classification： | Picornaviridae, Hepatovirus |
| Deposited As： | Hepatitis A |
| Agent： | Hepatitis A virus |
| Strain： | HAS-15 |
| Applications： | Enteric Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： | |
| Comments： | Genome contains 18-nucleotide deletion between VPI nucleotides 3383-2301. Attenuated for chimpanzees at 200 ng dose. |
| Effect on Host： | Gradual deterioration of cells; titer by ELISA or by RT-PCR (cells become persistently infected). Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate, but remain attached indicating persistent infection. |
| Recommended Host： | FRhK-4 (ATCC® CRL-1688™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 14-21 days at 37°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | BH Robertson |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Human feces |
| References： | Robertson BH, et al. Large scale production of hepatitis A virus in cell culture: effect of type of infection on virus yield and cell integrity. J. Gen. Virol. 69: 2129-2134, 1988. PubMed: 2841419 |
| Cross References： | Nucleotide (GenBank) : JQ425480.1 Hepatitis A virus strain HAS-15, complete genome |
