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Human coronavirus 229E
Human coronavirus 229E
规格:
货期:
编号:TS213598
品牌:Testobio
产品名称: Human coronavirus 229E
商品货号: TS213598
Classification: Coronaviridae, Coronavirus
Agent: Human coronavirus 229E
Strain: 229E
Common Name: Human coronavirus
Applications:
No hemolysis production.
Respiratory research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format: frozen 1 mL per vial
Storage Conditions: -70°C or colder
Comments:
Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.
Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). RU1 are human embryonic lung fibroblasts developed by CDC
Effect on Host:
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer.
Recommended Host:
MRC-5 (ATCC® CCL-171™)
Growth Conditions: Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended.
Name of Depositor: HS Kaye
Special Collection: ATCC
Source: Nasal and throat swabs from man with mild upper respiratory illness
References:

Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768

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Human coronavirus 229E

  • 货号: TS213598
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  • 品牌 : TESTOBIO
产品名称: Human coronavirus 229E
商品货号: TS213598
Classification: Coronaviridae, Coronavirus
Agent: Human coronavirus 229E
Strain: 229E
Common Name: Human coronavirus
Applications:
No hemolysis production.
Respiratory research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format: frozen 1 mL per vial
Storage Conditions: -70°C or colder
Comments:
Possibility of cross-reactions with other coronaviruses not thoroughly characterized at time of deposit (1973); does not cross-react with human myxoviruses. Ether sensitive. No hemolysis production. Unstable at 24 & 37C; relatively stable at 10C.
Harvest 26-50 hrs post adsorption before CPE is visible. Does not agglutinate chicken, guinea pig, human O, sheep, mouse or rat RBC. Size 89Mu (gradocol filtration). RU1 are human embryonic lung fibroblasts developed by CDC
Effect on Host:
CPE, rounding and sloughing. Starts as individual cell rounding that spreads, but over time uninfected cells repopulate monolayer.
Recommended Host:
MRC-5 (ATCC® CCL-171™)
Growth Conditions: Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 2-7 days, a 5% CO2 in air atmosphere is recommended.
Name of Depositor: HS Kaye
Special Collection: ATCC
Source: Nasal and throat swabs from man with mild upper respiratory illness
References:

Hamre D, Procknow JJ. A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med. 121: 190-193, 1966. PubMed: 4285768

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