| 产品名称： | Human adenovirus 42 |
|---|---|
| 商品货号： | TS213645 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus D |
| Agent： | Human adenovirus 42 |
| Strain： | 54/82 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | No cross-reactions by serum neutralization. Significant cross-reactions with Human adenovirus 15 and 22, bilaterally, by hemagglutinin inhibition assay. Hemagglutinates human or rhesus red blood cells. VR-1792™ was derived from VR-1304™ following treatment with diethyl ether to remove mycoplasma contamination. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.05). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days |
| Name of Depositor： | JC Hierholzer, Centers for Disease Control and Prevention; ATCC |
| Source： | Stool from an asymptomatic 1 year old male, Paris, 1982. Treated to remove mycoplasma at ATCC. |
| References： | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Wigand R, et al. A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch. Virol. 94: 283-286, 1987. PubMed: 3034212 |
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| 产品名称： | Human adenovirus 42 |
|---|---|
| 商品货号： | TS213645 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus D |
| Agent： | Human adenovirus 42 |
| Strain： | 54/82 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | No cross-reactions by serum neutralization. Significant cross-reactions with Human adenovirus 15 and 22, bilaterally, by hemagglutinin inhibition assay. Hemagglutinates human or rhesus red blood cells. VR-1792™ was derived from VR-1304™ following treatment with diethyl ether to remove mycoplasma contamination. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.05). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days |
| Name of Depositor： | JC Hierholzer, Centers for Disease Control and Prevention; ATCC |
| Source： | Stool from an asymptomatic 1 year old male, Paris, 1982. Treated to remove mycoplasma at ATCC. |
| References： | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Wigand R, et al. A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch. Virol. 94: 283-286, 1987. PubMed: 3034212 |
