| 产品名称： | Feline calicivirus |
|---|---|
| 商品货号： | TS213665 |
| Classification： | Caliciviridae, Vesivirus |
| Agent： | Feline calicivirus |
| Strain： | FCV-2280 |
| Applications： | The virus is not neutralized by FCV-M8 antisera. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genome Sequenced Strain： | Feline calicivirus strain FCV-2280, complete genome |
| Images： |  |
| Comments： | FCV-2280 is not neutralized by antiserum to the vaccine strains FCV-M8, FCV-F9 and FCV-255.xa0 This virus has been adapted to tissue culture propagation and may not readily infect cats.xa0 Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe on the patent claims. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： |
Fcwf-4 cells (ATCC® CRL-2787™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa02-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | University of California, Davis |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | Patent |
| Source： | Blood from naturally infected cat |
| References： | Pedersen NC, Laliberte L, and Ekman S. A transient febrile limping syndrome of kittens caused by two different strains of feline calicivirus. Feline Pract 13(1): 26-35, 1983. Pedersen NC. Feline calicivirus vaccine. US Patent 4,522,810 dated Jun 11 1985 |
| Cross References： | Nucleotide (GenBank) : KC835209.1 Feline calicivirus strain FCV-2280, complete genome |
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| 产品名称： | Feline calicivirus |
|---|---|
| 商品货号： | TS213665 |
| Classification： | Caliciviridae, Vesivirus |
| Agent： | Feline calicivirus |
| Strain： | FCV-2280 |
| Applications： | The virus is not neutralized by FCV-M8 antisera. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Genome Sequenced Strain： | Feline calicivirus strain FCV-2280, complete genome |
| Images： | |
| Comments： | FCV-2280 is not neutralized by antiserum to the vaccine strains FCV-M8, FCV-F9 and FCV-255.xa0 This virus has been adapted to tissue culture propagation and may not readily infect cats.xa0 Note: This material is cited in a U.S. and/or other Patent and may not be used to infringe on the patent claims. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： |
Fcwf-4 cells (ATCC® CRL-2787™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa02-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | University of California, Davis |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Special Collection： | Patent |
| Source： | Blood from naturally infected cat |
| References： | Pedersen NC, Laliberte L, and Ekman S. A transient febrile limping syndrome of kittens caused by two different strains of feline calicivirus. Feline Pract 13(1): 26-35, 1983. Pedersen NC. Feline calicivirus vaccine. US Patent 4,522,810 dated Jun 11 1985 |
| Cross References： | Nucleotide (GenBank) : KC835209.1 Feline calicivirus strain FCV-2280, complete genome |
