| 产品名称： | Human echovirus 9 |
|---|---|
| 商品货号： | TS213668 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus B |
| Deposited As： | Echovirus 9 |
| Agent： | Human echovirus 9 |
| Strain： | Hill |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Rectal swab from healthy child, Ohio |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | This strain is not pathogenic for newborn mice. Related antigenically to Coxsackievirus Type A23. Wigand, R. et al., Arch. Gesamte Virusforsch. 11: 708, 1962. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 LLC-MK2 (ATCC CCL-7) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent) for inoculation. |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | HA Wenner |
| Source： | Rectal swab from healthy child, Ohio |
| References： | Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336 Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578 Wigand R, Sabin AB. Antigenic purity and plaque properties of the prototype strains of ECHO virus types 7 to 11, and 17 and 18. Arch. Gesamte Virusforsch. 11: 708-717, 1962. PubMed: 14040255 or colder or colder |
| Cross References： | Nucleotide (GenBank) : U11710 Echovirus 9 Hill polyprotein gene, 5 UTR. |
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| 产品名称： | Human echovirus 9 |
|---|---|
| 商品货号： | TS213668 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus B |
| Deposited As： | Echovirus 9 |
| Agent： | Human echovirus 9 |
| Strain： | Hill |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Rectal swab from healthy child, Ohio |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | This strain is not pathogenic for newborn mice. Related antigenically to Coxsackievirus Type A23. Wigand, R. et al., Arch. Gesamte Virusforsch. 11: 708, 1962. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 LLC-MK2 (ATCC CCL-7) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent) for inoculation. |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | HA Wenner |
| Source： | Rectal swab from healthy child, Ohio |
| References： | Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336 Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578 Wigand R, Sabin AB. Antigenic purity and plaque properties of the prototype strains of ECHO virus types 7 to 11, and 17 and 18. Arch. Gesamte Virusforsch. 11: 708-717, 1962. PubMed: 14040255 or colder or colder |
| Cross References： | Nucleotide (GenBank) : U11710 Echovirus 9 Hill polyprotein gene, 5 UTR. |
