| 产品名称： | Human gammaherpesvirus 4 (HHV-4) |
|---|---|
| 商品货号： | TS213707 |
| Classification： | Herpesviridae, Lymphocryptovirus |
| Deposited As： | Burkitts lymphoma |
| Agent： | Human gammaherpesvirus 4 (HHV-4) |
| Strain： | HR1 (clone of P3) |
| Common Name： | Epstein-Barr virus |
| Applications： | The virus is not detected by PCR primers targeting EBNA-2, EBNA-3 regions or by immunofluorescence for anti-VCA and anti-EBNA. Virus was detected by monoclonal antibody against EBNA-1 from Chemicon cat# MAB8173. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | vapor phase of liquid nitrogen |
| Comments： | The cells are persistently infected with Human herpesvirus 4 (formerly Epstein-Barr virus, EBV). The HR-1 strain of Epstein-Barr virus (EBV) has a deletion in the EBNA2 gene. The virus is not detected by PCR primers targeting EBNA-2, EBNA-3 regions or by immunofluorescence for anti-VCA and anti-EBNA. Virus was detected by monoclonal antibody against EBNA-1 from Chemicon cat# MAB8173. |
| Effect on Host： | Yes, in vitro effects: Polykaryocyte formation, cell swelling Polykaryocyte formation, cell swelling |
| Recommended Host： | Host of Choice: Human lymphoblastoid cells Host Range: Human lymphoblastoid cells (NC37, F265) |
| Growth Conditions： | Temperature: 37.0°C Max Temperature: 37.0°C Min Temperature: 35.0°C Duration: 5-12 days Protocol: Thaw rapidly, resuspend the ampule contents in 10 ml of cold medium, centrifuge at 800 X g for 5 min, remove the supernatant and resuspend the cells in fetal bovine serum-suplemented medium in a small culture flask filled to a depth of 2 to 3 mm. |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Subcultivation： | Protocol: Thaw rapidly, resuspend the ampule contents in 10 ml of cold medium, centrifuge at 800 X g for 5 min, remove the supernatant and resuspend the cells in fetal bovine serum-suplemented medium in a small culture flask filled to a depth of 2 to 3 mm. |
| Name of Depositor： | FT Buscheck |
| Special Collection： | ATCC |
| References： | Hinuma Y, Grace JT Jr.. Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc. Soc. Exp. Biol. Med. 124: 107-111, 1967. PubMed: 4163469 This virus is continuously produced by the cell line in which it is shipped. No additional host is required, however, if desired the virus may be used to infect other cells which are listed as alternate hosts. |
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| 产品名称： | Human gammaherpesvirus 4 (HHV-4) |
|---|---|
| 商品货号： | TS213707 |
| Classification： | Herpesviridae, Lymphocryptovirus |
| Deposited As： | Burkitts lymphoma |
| Agent： | Human gammaherpesvirus 4 (HHV-4) |
| Strain： | HR1 (clone of P3) |
| Common Name： | Epstein-Barr virus |
| Applications： | The virus is not detected by PCR primers targeting EBNA-2, EBNA-3 regions or by immunofluorescence for anti-VCA and anti-EBNA. Virus was detected by monoclonal antibody against EBNA-1 from Chemicon cat# MAB8173. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | vapor phase of liquid nitrogen |
| Comments： | The cells are persistently infected with Human herpesvirus 4 (formerly Epstein-Barr virus, EBV). The HR-1 strain of Epstein-Barr virus (EBV) has a deletion in the EBNA2 gene. The virus is not detected by PCR primers targeting EBNA-2, EBNA-3 regions or by immunofluorescence for anti-VCA and anti-EBNA. Virus was detected by monoclonal antibody against EBNA-1 from Chemicon cat# MAB8173. |
| Effect on Host： | Yes, in vitro effects: Polykaryocyte formation, cell swelling Polykaryocyte formation, cell swelling |
| Recommended Host： | Host of Choice: Human lymphoblastoid cells Host Range: Human lymphoblastoid cells (NC37, F265) |
| Growth Conditions： | Temperature: 37.0°C Max Temperature: 37.0°C Min Temperature: 35.0°C Duration: 5-12 days Protocol: Thaw rapidly, resuspend the ampule contents in 10 ml of cold medium, centrifuge at 800 X g for 5 min, remove the supernatant and resuspend the cells in fetal bovine serum-suplemented medium in a small culture flask filled to a depth of 2 to 3 mm. |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Subcultivation： | Protocol: Thaw rapidly, resuspend the ampule contents in 10 ml of cold medium, centrifuge at 800 X g for 5 min, remove the supernatant and resuspend the cells in fetal bovine serum-suplemented medium in a small culture flask filled to a depth of 2 to 3 mm. |
| Name of Depositor： | FT Buscheck |
| Special Collection： | ATCC |
| References： | Hinuma Y, Grace JT Jr.. Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc. Soc. Exp. Biol. Med. 124: 107-111, 1967. PubMed: 4163469 This virus is continuously produced by the cell line in which it is shipped. No additional host is required, however, if desired the virus may be used to infect other cells which are listed as alternate hosts. |
