| 产品名称： | Human adenovirus 11 |
|---|---|
| 商品货号： | TS213722 |
| Classification： | Adenoviridae, Mastadenovirus, Human mastadenovirus B |
| Deposited As： | Adenovirus type 11 |
| Agent： | Human adenovirus 11 |
| Strain： | Slobitski |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Agglutinates rhesus RBC.xa0 Weakly oncogenic.xa0 Contains soluble group-specific CF antigen.xa0 Efforts at ATCC® to detect polio by antibody-antigen reaction in VR-12™ have yielded negative results. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | KB cells (ATCC® CCL-17™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | M Schaeffer, DA Stevens |
| Special Collection： | ATCC |
| Source： | Feces, human, Massachusetts, United States |
| References： | Rowe WP, Hartley JW, Huebner RJ. Additional serotypes of the APC virus group. Proc. Soc. Exp. Biol. Med. 91: 260-262, 1956. PubMed: 13297771 Kibrick S, Melendez L, Enders JF. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. NY Acad. Sci. 67: 311-325, 1957. PubMed: 13411969 Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 Feces from patient with paralytic poliomyelitis, Massachusetts. Type 1 poliovirus also isolated. |
| Cross References： | Nucleotide (GenBank) : U52569 Human adenovirus type 11 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds. |
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| 产品名称： | Human adenovirus 11 |
|---|---|
| 商品货号： | TS213722 |
| Classification： | Adenoviridae, Mastadenovirus, Human mastadenovirus B |
| Deposited As： | Adenovirus type 11 |
| Agent： | Human adenovirus 11 |
| Strain： | Slobitski |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Agglutinates rhesus RBC.xa0 Weakly oncogenic.xa0 Contains soluble group-specific CF antigen.xa0 Efforts at ATCC® to detect polio by antibody-antigen reaction in VR-12™ have yielded negative results. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | KB cells (ATCC® CCL-17™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | M Schaeffer, DA Stevens |
| Special Collection： | ATCC |
| Source： | Feces, human, Massachusetts, United States |
| References： | Rowe WP, Hartley JW, Huebner RJ. Additional serotypes of the APC virus group. Proc. Soc. Exp. Biol. Med. 91: 260-262, 1956. PubMed: 13297771 Kibrick S, Melendez L, Enders JF. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. NY Acad. Sci. 67: 311-325, 1957. PubMed: 13411969 Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 Feces from patient with paralytic poliomyelitis, Massachusetts. Type 1 poliovirus also isolated. |
| Cross References： | Nucleotide (GenBank) : U52569 Human adenovirus type 11 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds. |
