| 产品名称： | Francisella persica Larson |
|---|---|
| 商品货号： | TS213769 |
| Classification： | Thiotrichales, Francisellaceae |
| Deposited As： | Wolbachia persica Suitor and Weiss |
| Agent： | Francisella persica Larson |
| Strain： | Original |
| Applications： | Vector-borne pathogens research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of egg culture product ATCC® VR-331™ to Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™). ATCC® VR-331™ and ATCC® VR-1853™ have not been compared for sequence or infectivity in egg culture and tissue culture. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™) |
| Growth Conditions： | Temperature: 28°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 28°C in a humidified, air atmosphere incubator. End adsorption by adding virus growth medium. Incubation: 6-8 days at 28°C in a humidified, air atmosphere incubator, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | E Weiss, Naval Medical Research Center |
| Source： | Malpighian tubules of ticks, Argas persicus (Oken) (now Argas (Persicargas) arboreus), removed from buff-backed heron (Bubulcus ibis), U.A.R. |
| References： | Suitor EC, Weiss E. Isolation of a rickettsialike microorganism (Wolbachia persica n. sp.) from Argas persicus (Oken). J. Infect. Dis. 108: 95-106, 1961. Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Larson MA, et al. Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. Int. J. Syst. Evol. Microbiol. 66(3): 1200-1205, 2016. PubMed: 26747442 |
| Cross References： | Nucleotide (GenBank) : M21292 Wolbachia persica 16S ribosomal RNA. |
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| 产品名称： | Francisella persica Larson |
|---|---|
| 商品货号： | TS213769 |
| Classification： | Thiotrichales, Francisellaceae |
| Deposited As： | Wolbachia persica Suitor and Weiss |
| Agent： | Francisella persica Larson |
| Strain： | Original |
| Applications： | Vector-borne pathogens research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Derived by adaptation of egg culture product ATCC® VR-331™ to Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™). ATCC® VR-331™ and ATCC® VR-1853™ have not been compared for sequence or infectivity in egg culture and tissue culture. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™) |
| Growth Conditions： | Temperature: 28°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 28°C in a humidified, air atmosphere incubator. End adsorption by adding virus growth medium. Incubation: 6-8 days at 28°C in a humidified, air atmosphere incubator, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | E Weiss, Naval Medical Research Center |
| Source： | Malpighian tubules of ticks, Argas persicus (Oken) (now Argas (Persicargas) arboreus), removed from buff-backed heron (Bubulcus ibis), U.A.R. |
| References： | Suitor EC, Weiss E. Isolation of a rickettsialike microorganism (Wolbachia persica n. sp.) from Argas persicus (Oken). J. Infect. Dis. 108: 95-106, 1961. Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Larson MA, et al. Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. Int. J. Syst. Evol. Microbiol. 66(3): 1200-1205, 2016. PubMed: 26747442 |
| Cross References： | Nucleotide (GenBank) : M21292 Wolbachia persica 16S ribosomal RNA. |
