| 产品名称： | Human gammaherpesvirus 4 (HHV-4) |
|---|---|
| 商品货号： | TS213775 |
| Classification： | Herpesviridae, Lymphocryptovirus |
| Deposited As： | Epstein-Barr virus |
| Agent： | Human gammaherpesvirus 4 (HHV-4) |
| Strain： | B95-8 |
| Common Name： | Epstein-Barr virus |
| Applications： | Human and other primate B-lymphocyte transformation In vitroxa0infectivity assay Antiviral Studies Spiking studies ELISpot Assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | ATCC® VR-1492PQ™ is a high-titer, purified, live virus suspended in 10 mM Tris-HCL (pH 7.5) with 135 mM NaCl, 0.5% BSA, and 5% Trehalose. This product was prepared from ATCC® VR-1492™ via purification by tangential filtration. This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). Quality control testing is done by transformation assay instead of TCID50. |
| Effect on Host： | EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells. |
| Recommended Host： | B95-8 cells |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). Incubation: 10-25 days at 37°C in a humidified 5% CO2 atmosphere, until indicators of successful transformation appear. |
| Name of Depositor： | G Miller |
| Source： | Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis. |
| References： | Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991. Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982 Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497 |
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| 产品名称： | Human gammaherpesvirus 4 (HHV-4) |
|---|---|
| 商品货号： | TS213775 |
| Classification： | Herpesviridae, Lymphocryptovirus |
| Deposited As： | Epstein-Barr virus |
| Agent： | Human gammaherpesvirus 4 (HHV-4) |
| Strain： | B95-8 |
| Common Name： | Epstein-Barr virus |
| Applications： | Human and other primate B-lymphocyte transformation In vitroxa0infectivity assay Antiviral Studies Spiking studies ELISpot Assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | ATCC® VR-1492PQ™ is a high-titer, purified, live virus suspended in 10 mM Tris-HCL (pH 7.5) with 135 mM NaCl, 0.5% BSA, and 5% Trehalose. This product was prepared from ATCC® VR-1492™ via purification by tangential filtration. This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). Quality control testing is done by transformation assay instead of TCID50. |
| Effect on Host： | EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells. |
| Recommended Host： | B95-8 cells |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™). Incubation: 10-25 days at 37°C in a humidified 5% CO2 atmosphere, until indicators of successful transformation appear. |
| Name of Depositor： | G Miller |
| Source： | Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis. |
| References： | Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991. Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982 Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497 |
