| 产品名称： | Mumps rubulavirus |
|---|---|
| 商品货号： | TS213781 |
| Classification： | Paramyxoviridae, Rubulavirus |
| Deposited As： | Mumps |
| Agent： | Mumps rubulavirus |
| Strain： | Torii |
| Common Name： | Mumps virus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-638™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1880™ and ATCC® VR-638™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. |
| Effect on Host： | CPE, syncytia with cell degeneration and sloughing |
| Recommended Host： | Vero cells (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01-0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | Takeda Chem. Ind., Ltd. |
| Source： | Throat swab from 5-year-old male with mumps, Japan, 1969 |
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| 产品名称： | Mumps rubulavirus |
|---|---|
| 商品货号： | TS213781 |
| Classification： | Paramyxoviridae, Rubulavirus |
| Deposited As： | Mumps |
| Agent： | Mumps rubulavirus |
| Strain： | Torii |
| Common Name： | Mumps virus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Derived by adaptation of primary cell culture product ATCC® VR-638™ to Vero cells (ATCC® CCL-81™). ATCC® VR-1880™ and ATCC® VR-638™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. |
| Effect on Host： | CPE, syncytia with cell degeneration and sloughing |
| Recommended Host： | Vero cells (ATCC® CCL-81™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01-0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 4-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | Takeda Chem. Ind., Ltd. |
| Source： | Throat swab from 5-year-old male with mumps, Japan, 1969 |
