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Usutu virus
Usutu virus
规格:
货期:
编号:TS213790
品牌:Testobio
产品名称: Usutu virus
商品货号: TS213790
Classification: Flaviviridae, Flavivirus
Agent: Usutu virus
Strain: SAAR 1776
Applications: Vector-borne disease research
Emerging infectious disease research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format: frozen 1.0 mL per vial
Storage Conditions: -70°C or colder
Images: VR-1891 images
Comments: Arbovirus
Effect on Host:
CPE, refractile rounding and sloughing
Recommended Host:
Vero cells (ATCC® CCL-81™)
Growth Conditions:

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor: B Russell, CDC
Source: Isolated from a mosquito in Ndumu, Natal, South Africa, 1959
References:

Ashraf U, et al. Usutu Virus: An Emerging Flavivirus in Europe. Viruses 7: 219-238, 2015. PubMed: 25606971

Tetro JA. Is Usutu Virus Ready for Prime Time? Microbes Infect 19: 380-381, 2017. PubMed: 28619684

Pecorari M, et al. First Human Case of Usutu Virus Neuroinvasive Infection, Italy, August-September 2009. Euro Surveill 14(50): 19446, 2009. PubMed: 20070936

Santini M, et al. First Cases of Human Usutu Virus Neuroinvasive Infection in Croatia, August-September 2013: Clinical and Laboratory Features. J Neurovirol 21: 92-97, 2015. PubMed: 25361698

Gaibani P, et al. Comparative Genomic and Phylogenetic Analysis of the Usutu Virus Isolate from a Human Patient Presenting with Neurological Symptoms. PLoS One 8: e64761, 2013. PubMed: 23741387

Williams MC, et al. The Isolation of West Nile Virus from Man and of Usutu Virus from the Bird-Biting Mosquito Mansonia aurites (Theobald) in the Entebbe Area of Uganda. Ann Trop Med Parasitol 58: 367-374, 1964. PubMed: 14212897

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Usutu virus

  • 货号: TS213790
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: Usutu virus
商品货号: TS213790
Classification: Flaviviridae, Flavivirus
Agent: Usutu virus
Strain: SAAR 1776
Applications: Vector-borne disease research
Emerging infectious disease research
Biosafety Level: 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format: frozen 1.0 mL per vial
Storage Conditions: -70°C or colder
Images: VR-1891 images
Comments: Arbovirus
Effect on Host:
CPE, refractile rounding and sloughing
Recommended Host:
Vero cells (ATCC® CCL-81™)
Growth Conditions:

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor: B Russell, CDC
Source: Isolated from a mosquito in Ndumu, Natal, South Africa, 1959
References:

Ashraf U, et al. Usutu Virus: An Emerging Flavivirus in Europe. Viruses 7: 219-238, 2015. PubMed: 25606971

Tetro JA. Is Usutu Virus Ready for Prime Time? Microbes Infect 19: 380-381, 2017. PubMed: 28619684

Pecorari M, et al. First Human Case of Usutu Virus Neuroinvasive Infection, Italy, August-September 2009. Euro Surveill 14(50): 19446, 2009. PubMed: 20070936

Santini M, et al. First Cases of Human Usutu Virus Neuroinvasive Infection in Croatia, August-September 2013: Clinical and Laboratory Features. J Neurovirol 21: 92-97, 2015. PubMed: 25361698

Gaibani P, et al. Comparative Genomic and Phylogenetic Analysis of the Usutu Virus Isolate from a Human Patient Presenting with Neurological Symptoms. PLoS One 8: e64761, 2013. PubMed: 23741387

Williams MC, et al. The Isolation of West Nile Virus from Man and of Usutu Virus from the Bird-Biting Mosquito Mansonia aurites (Theobald) in the Entebbe Area of Uganda. Ann Trop Med Parasitol 58: 367-374, 1964. PubMed: 14212897

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