| 产品名称： | Macaca mulatta polyomavirus 1 |
|---|---|
| 商品货号： | TS213798 |
| Classification： | Polyomaviridae, Betapolyomavirus |
| Deposited As： | Macaca mulatta polyomavirus 1 |
| Agent： | Macaca mulatta polyomavirus 1 |
| Strain： | 776 776-2E (WT) |
| Common Name： | Simian virus 40, SV40 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Effect on Host： | CPE, vacuolization, rounding, and sloughing |
| Recommended Host： | CV-1 cells (ATCC® CCL-70™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Butel, Baylor College of Medicine |
| Source： | Isolated from an Adenovirus type 1 seed stock, 1960. |
| References： | Sweet BH and Hilleman MR.xa0 The vacuolating virus, S.V.40. Proc Soc Exp Biol Med 105:420-427, 1960. PubMed: 13774265 Lednicky J and Folk WR. Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J Virol 66(11): 6379-6390, 1992. PubMed: 1328672 Stewart AR, et al.xa0 Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221(2):355-361, 1996. PubMed: 8661447 Forsman ZH, et al.xa0 Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.xa0 J Virol 78(17): 9306-9316, 2004.xa0 PubMed: 15308725 Swain JL, et al.xa0 Effects of route of inoculation and viral genetic variation on antibody responses to polyomavirus SV40 in Syrian golden hamsters. Comp Med 62(5):400-408, 2012. PubMed: 23114044 Zhang S, et al.xa0 Viral microRNA effects on pathogenesis of polyomavirus SV40 infections in Syrian golden hamsters.xa0PLoS Pathog 10(2):e1003912, 2004.xa0PubMed: 24516384 |
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| 产品名称： | Macaca mulatta polyomavirus 1 |
|---|---|
| 商品货号： | TS213798 |
| Classification： | Polyomaviridae, Betapolyomavirus |
| Deposited As： | Macaca mulatta polyomavirus 1 |
| Agent： | Macaca mulatta polyomavirus 1 |
| Strain： | 776 776-2E (WT) |
| Common Name： | Simian virus 40, SV40 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Effect on Host： | CPE, vacuolization, rounding, and sloughing |
| Recommended Host： | CV-1 cells (ATCC® CCL-70™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Butel, Baylor College of Medicine |
| Source： | Isolated from an Adenovirus type 1 seed stock, 1960. |
| References： | Sweet BH and Hilleman MR.xa0 The vacuolating virus, S.V.40. Proc Soc Exp Biol Med 105:420-427, 1960. PubMed: 13774265 Lednicky J and Folk WR. Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J Virol 66(11): 6379-6390, 1992. PubMed: 1328672 Stewart AR, et al.xa0 Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221(2):355-361, 1996. PubMed: 8661447 Forsman ZH, et al.xa0 Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.xa0 J Virol 78(17): 9306-9316, 2004.xa0 PubMed: 15308725 Swain JL, et al.xa0 Effects of route of inoculation and viral genetic variation on antibody responses to polyomavirus SV40 in Syrian golden hamsters. Comp Med 62(5):400-408, 2012. PubMed: 23114044 Zhang S, et al.xa0 Viral microRNA effects on pathogenesis of polyomavirus SV40 infections in Syrian golden hamsters.xa0PLoS Pathog 10(2):e1003912, 2004.xa0PubMed: 24516384 |
