| 产品名称： | Epizootic hemorrhagic disease virus 2 |
|---|---|
| 商品货号： | TS213799 |
| Classification： | Sedoreovirinae, Orbivirus, Epizootic hemorrhagic disease virus |
| Agent： | Epizootic hemorrhagic disease virus 2 |
| Strain： | OV215 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Strain fully sequenced by depositor |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Loeb, University of Florida |
| Chain of Custody： | ATCC |
| Source： | Spleen of a farmed white-tailed deer, Gadsden County, Florida, USA. Isolated on October 11, 2016. |
| Year of Origin： | 2016 |
| References： | Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661 Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175 |
| Cross References： | Nucleotide (GenBank) : MF688816.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP1 protein (VP1) gene, complete cds. Nucleotide (GenBank) : MF688817.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP2 protein (VP2) gene, complete cds. Nucleotide (GenBank) : MF688818.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP3 protein (VP3) gene, complete cds. Nucleotide (GenBank) : MF688819.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP4 protein (VP4) gene, complete cds. Nucleotide (GenBank) : MF688820.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP5 protein (VP5) gene, complete cds. Nucleotide (GenBank) : MF688821.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP6 protein (VP6) gene, complete cds. Nucleotide (GenBank) : MF688822.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP7 protein (VP7) gene, complete cds. Nucleotide (GenBank) : MF688823.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS1 protein (NS1) gene, complete cds. Nucleotide (GenBank) : MF688824.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS2 protein (NS2) gene, complete cds. Nucleotide (GenBank) : MF688825.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS3 protein (NS3) gene, complete cds. |
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| 产品名称： | Epizootic hemorrhagic disease virus 2 |
|---|---|
| 商品货号： | TS213799 |
| Classification： | Sedoreovirinae, Orbivirus, Epizootic hemorrhagic disease virus |
| Agent： | Epizootic hemorrhagic disease virus 2 |
| Strain： | OV215 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Strain fully sequenced by depositor |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Loeb, University of Florida |
| Chain of Custody： | ATCC |
| Source： | Spleen of a farmed white-tailed deer, Gadsden County, Florida, USA. Isolated on October 11, 2016. |
| Year of Origin： | 2016 |
| References： | Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661 Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175 |
| Cross References： | Nucleotide (GenBank) : MF688816.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP1 protein (VP1) gene, complete cds. Nucleotide (GenBank) : MF688817.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP2 protein (VP2) gene, complete cds. Nucleotide (GenBank) : MF688818.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP3 protein (VP3) gene, complete cds. Nucleotide (GenBank) : MF688819.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP4 protein (VP4) gene, complete cds. Nucleotide (GenBank) : MF688820.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP5 protein (VP5) gene, complete cds. Nucleotide (GenBank) : MF688821.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP6 protein (VP6) gene, complete cds. Nucleotide (GenBank) : MF688822.1 Epizootic hemorrhagic disease virus 2 isolate OV215 VP7 protein (VP7) gene, complete cds. Nucleotide (GenBank) : MF688823.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS1 protein (NS1) gene, complete cds. Nucleotide (GenBank) : MF688824.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS2 protein (NS2) gene, complete cds. Nucleotide (GenBank) : MF688825.1 Epizootic hemorrhagic disease virus 2 isolate OV215 NS3 protein (NS3) gene, complete cds. |
