| 产品名称： | Human herpesvirus 5 |
|---|---|
| 商品货号： | TS213827 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Deposited As： | Cytomegalovirus |
| Agent： | Human herpesvirus 5 |
| Strain： | AD-169 |
| Common Name： | Human cytomegalovirus |
| Applications： | Testing virucides Sexually Transmitted Disease Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Genome Sequenced Strain： | Human cytomegalovirus strain AD169 complete genome |
| Images： |  |
| Comments： | Preserved in virus growth medium with 7% DMSO.
GenBank: X17403. |
| Effect on Host： | CPE, cell enlarging, rounding, sloughing |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.5 - 2.0 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1 to 1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation. Incubation: 4 to 11 days |
| Name of Depositor： | WA Chappell |
| Special Collection： | NCRR Contract |
| Source： | Adenoid tissue from 7-year-old female |
| References： | ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). Huang L, et al. The variable e endds of a human cytomegalovirus oriLyt transcript (SRT) overlap an essential, conserved replicator element. J. Virol. 70: 5272-5281, 1996. PubMed: 8764037 Iskenderian AC, et al. Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes. J. Virol. 70: 383-392, 1996. PubMed: 8523552 Noraz N, et al. Human cytomegalovirus-associated immunosuppression is mediated through interferon-alpha. Blood 89: 2443-2452, 1997. PubMed: 9116288 Prichard MN, et al. Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication. J. Virol. 70: 3018-3025, 1996. PubMed: 8627778 Rowe WP, et al. Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proc. Soc. Exp. Biol. Med. 92: 418-424, 1956. PubMed: 13350367 |
| Cross References： | Nucleotide (GenBank) : X17403 Human cytomegalovirus strain AD169 complete genome |
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| 产品名称： | Human herpesvirus 5 |
|---|---|
| 商品货号： | TS213827 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Deposited As： | Cytomegalovirus |
| Agent： | Human herpesvirus 5 |
| Strain： | AD-169 |
| Common Name： | Human cytomegalovirus |
| Applications： | Testing virucides Sexually Transmitted Disease Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Genome Sequenced Strain： | Human cytomegalovirus strain AD169 complete genome |
| Images： | |
| Comments： | Preserved in virus growth medium with 7% DMSO.
GenBank: X17403. |
| Effect on Host： | CPE, cell enlarging, rounding, sloughing |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.5 - 2.0 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1 to 1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation. Incubation: 4 to 11 days |
| Name of Depositor： | WA Chappell |
| Special Collection： | NCRR Contract |
| Source： | Adenoid tissue from 7-year-old female |
| References： | ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002). ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002). Huang L, et al. The variable e endds of a human cytomegalovirus oriLyt transcript (SRT) overlap an essential, conserved replicator element. J. Virol. 70: 5272-5281, 1996. PubMed: 8764037 Iskenderian AC, et al. Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes. J. Virol. 70: 383-392, 1996. PubMed: 8523552 Noraz N, et al. Human cytomegalovirus-associated immunosuppression is mediated through interferon-alpha. Blood 89: 2443-2452, 1997. PubMed: 9116288 Prichard MN, et al. Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication. J. Virol. 70: 3018-3025, 1996. PubMed: 8627778 Rowe WP, et al. Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proc. Soc. Exp. Biol. Med. 92: 418-424, 1956. PubMed: 13350367 |
| Cross References： | Nucleotide (GenBank) : X17403 Human cytomegalovirus strain AD169 complete genome |
