| 产品名称： | Human herpesvirus 5 |
|---|---|
| 商品货号： | TS213829 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Agent： | Human herpesvirus 5 |
| Strain： | Merlin |
| Common Name： | Human cytomegalovirus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Human herpesvirus 5 strain Merlin, complete genome |
| Comments： | Low MOI applications, such as titration, may require incubation for as long as 3 weeks. This is a low passage strain of Human herpesvirus 5, deposited at ATCC® 4 passages from isolation. At deposit, it retained the wild type complement of 165 genes, and contained no obvious mutations other than a single nucleotide substitution that truncates gene UL128. To retain genetic integrity, further passage should be limited. |
| Effect on Host： | CPE, cell rounding and enlargement, cytoplasmic and nuclear inclusions |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | GWG Wilkinson, Cardiff Public Health Laboratory |
| Source： | Urine from a congenitally infected infant, Cardiff, Wales |
| References： | Davison A J, et al. Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J. Gen. Virol. 84 (Pt 3), 657-663 (2003). PubMed: 12604818 Dolan A, et al. Genetic content of wild-type human cytomegalovirus. Journal of General Virology, 85: 1301-1312, 2004. PubMed: 15105547 |
| Cross References： | Nucleotide (GenBank) : AY446894.2 Human herpesvirus 5 strain Merlin, complete genome |
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| 产品名称： | Human herpesvirus 5 |
|---|---|
| 商品货号： | TS213829 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Agent： | Human herpesvirus 5 |
| Strain： | Merlin |
| Common Name： | Human cytomegalovirus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Human herpesvirus 5 strain Merlin, complete genome |
| Comments： | Low MOI applications, such as titration, may require incubation for as long as 3 weeks. This is a low passage strain of Human herpesvirus 5, deposited at ATCC® 4 passages from isolation. At deposit, it retained the wild type complement of 165 genes, and contained no obvious mutations other than a single nucleotide substitution that truncates gene UL128. To retain genetic integrity, further passage should be limited. |
| Effect on Host： | CPE, cell rounding and enlargement, cytoplasmic and nuclear inclusions |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | GWG Wilkinson, Cardiff Public Health Laboratory |
| Source： | Urine from a congenitally infected infant, Cardiff, Wales |
| References： | Davison A J, et al. Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J. Gen. Virol. 84 (Pt 3), 657-663 (2003). PubMed: 12604818 Dolan A, et al. Genetic content of wild-type human cytomegalovirus. Journal of General Virology, 85: 1301-1312, 2004. PubMed: 15105547 |
| Cross References： | Nucleotide (GenBank) : AY446894.2 Human herpesvirus 5 strain Merlin, complete genome |
