pMAP517 [112-12D]
pMAP517 [112-12D]
规格:
货期:
编号:TS213901
品牌:Testobio
| 产品名称： | pMAP517 112-12D |
|---|---|
| 商品货号： | TS213901 |
| Designations： | pMAP517 112-12D |
| Species： | Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors： | Monsanto Co., FJ Perlak, Monsanto Co. |
| Applications： | produces protein crystal protein (delta endotoxin) |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pMAP515 Intact vector size: Type of vector: plasmid Vector end: BglII Vector end: BglII Cloning sites: BglII Polylinker sites: Other unique sites: XhoI Construction: pSUP1011, pMON7068, pMAP33 Features (with orientation and position when available): |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: Y Strain: HD-1 Type of DNA: plasmid Insert 5 end: BamHI Insert 3 end: BamHI Modification: BamHI -> BglII Insert size (kb): 4.6 Cross references: Insert lengths(kb): 4.599999904632568 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 4.600 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The IS50L(kanR/B.t.) gene segment was integrated into the host chromosome by mating E. coli S17-1 containing pMAP517 with P. fluorescens 112-12. The IS50L(kanR/B.t.) gene segment has a +4 frameshift mutation (which converts a wild type XhoI site to a PvuI site) of the transposase gene IS50L. This strain is resistant to 100 mcg/ml rifampicin and 100 mcg/ml nalidixic acid. Strain that proliferates in a plant environment to an extent sufficient to express at least 0.5 ng of insecticidally active protein per each insect to be controlled. |
| References： | Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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pMAP517 [112-12D]
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| 产品名称： | pMAP517 112-12D |
|---|---|
| 商品货号： | TS213901 |
| Designations： | pMAP517 112-12D |
| Species： | Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors： | Monsanto Co., FJ Perlak, Monsanto Co. |
| Applications： | produces protein crystal protein (delta endotoxin) |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pMAP515 Intact vector size: Type of vector: plasmid Vector end: BglII Vector end: BglII Cloning sites: BglII Polylinker sites: Other unique sites: XhoI Construction: pSUP1011, pMON7068, pMAP33 Features (with orientation and position when available): |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Bacillus thuringiensis subsp. kurstaki Gene name: crystal protein (delta endotoxin) Contains complete coding sequence?: Y Strain: HD-1 Type of DNA: plasmid Insert 5 end: BamHI Insert 3 end: BamHI Modification: BamHI -> BglII Insert size (kb): 4.6 Cross references: Insert lengths(kb): 4.599999904632568 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb)： | 4.600 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The IS50L(kanR/B.t.) gene segment was integrated into the host chromosome by mating E. coli S17-1 containing pMAP517 with P. fluorescens 112-12. The IS50L(kanR/B.t.) gene segment has a +4 frameshift mutation (which converts a wild type XhoI site to a PvuI site) of the transposase gene IS50L. This strain is resistant to 100 mcg/ml rifampicin and 100 mcg/ml nalidixic acid. Strain that proliferates in a plant environment to an extent sufficient to express at least 0.5 ng of insecticidally active protein per each insect to be controlled. |
| References： | Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
