| 产品名称： | Aw3.18.14 |
|---|---|
| 商品货号： | TS213929 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte; somatic cell hybrid |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | CAF1 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Animals were immunized with M12-I-Ak 48-62 cells.xa0Ref Spleen cells were fused with P3X63Ag8 myeloma cells.xa0 |
| Comments： | The antibody recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-Ak. |
| Complete Growth Medium： | Dulbeccos Modified Eagles Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Shake off the attached cells and transfer along with the floating cells into new flasks. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 x 104xa0viable cells/mL.xa0Maintain cell density between 2 x 104xa0and 2 x 105xa0viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO.xa0Cell culture tested DMSO is available as ATCC®xa0Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C |
| Isotype： | mouse IgG1 kappa |
| Population Doubling Time： | 14 hours |
| Name of Depositor： | ER Unanue |
| Year of Origin： | 1996 |
| References： | Dadaglio G, et al. Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody. Immunity 6: 727-738, 1997. PubMed: 9208845 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
Dadaglio G, et al. Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody. Immunity 6: 727-738, 1997. PubMed: 9208845
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| 产品名称： | Aw3.18.14 |
|---|---|
| 商品货号： | TS213929 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte; somatic cell hybrid |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | CAF1 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： | |
| Derivation： | Animals were immunized with M12-I-Ak 48-62 cells.xa0Ref Spleen cells were fused with P3X63Ag8 myeloma cells.xa0 |
| Comments： | The antibody recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-Ak. |
| Complete Growth Medium： | Dulbeccos Modified Eagles Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Shake off the attached cells and transfer along with the floating cells into new flasks. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 x 104xa0viable cells/mL.xa0Maintain cell density between 2 x 104xa0and 2 x 105xa0viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO.xa0Cell culture tested DMSO is available as ATCC®xa0Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C |
| Isotype： | mouse IgG1 kappa |
| Population Doubling Time： | 14 hours |
| Name of Depositor： | ER Unanue |
| Year of Origin： | 1996 |
| References： | Dadaglio G, et al. Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody. Immunity 6: 727-738, 1997. PubMed: 9208845 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
