IMR-90

规格:

货期:

编号:TS213976

品牌:Testobio

产品名称：	IMR-90
商品货号：	TS213976
Organism：	Homo sapiens, human
Tissue：	lung
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	16 weeks gestation
Gender：	female
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	normal human female; diploid; stable. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images：
Derivation：	The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus.
Clinical Data：	16 weeks gestation Caucasian female
Virus Susceptibility：	Human poliovirus 1 Human poliovirus 2 Varicella-Zoster Herpes simplex virus 1 Herpes simplex virus 2 Human poliovirus 3 Vaccinia virus Human herpesvirus 5 , Human cytomegalovirus Vesicular stomatitis virus
Comments：	The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 3 to 4 days
Cryopreservation：	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 10,13 D5S818: 12,13 D7S820: 9,12 THO1: 8,9.3 TPOX: 8,9 vWA: 16,19
Isoenzymes：	G6PD, B
Name of Depositor：	WW Nichols
Deposited As：	Homo sapiens
References：	Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339 Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642 Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

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IMR-90

货号: TS213976
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产品名称：	IMR-90
商品货号：	TS213976
Organism：	Homo sapiens, human
Tissue：	lung
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	16 weeks gestation
Gender：	female
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	normal human female; diploid; stable. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images：
Derivation：	The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus.
Clinical Data：	16 weeks gestation Caucasian female
Virus Susceptibility：	Human poliovirus 1 Human poliovirus 2 Varicella-Zoster Herpes simplex virus 1 Herpes simplex virus 2 Human poliovirus 3 Vaccinia virus Human herpesvirus 5 , Human cytomegalovirus Vesicular stomatitis virus
Comments：	The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 3 to 4 days
Cryopreservation：	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 10,13 D5S818: 12,13 D7S820: 9,12 THO1: 8,9.3 TPOX: 8,9 vWA: 16,19
Isoenzymes：	G6PD, B
Name of Depositor：	WW Nichols
Deposited As：	Homo sapiens
References：	Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339 Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642 Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564