| 产品名称： | SCC-15 |
|---|---|
| 商品货号： | TS214044 |
| Organism： | Homo sapiens, human |
| Tissue： | tongue |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 55 years |
| Gender： | male |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Clinical Data： | male
55 years |
| Genes Expressed： | epidermal keratins; 40 kD keratin; low levels of involucrin |
| Cellular Products： | epidermal keratins; 40 kD keratin; low levels of involucrin |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | Unlike SCC-4 (ATCC CRL-1624), this line does not grow well in semi-solid medium, and does not require a feeder layer. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,14 D16S539: 12,15 D5S818: 12 D7S820: 10,11 THO1: 9,9.3 TPOX: 8 vWA: 15,17 |
| Name of Depositor： | JG Rheinwald |
| Deposited As： | Homo sapiens |
| References： | Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336 Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916 Wu YJ, Rheinwald JG. A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Cell 25: 627-635, 1981. PubMed: 6169443 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SCC-15 |
|---|---|
| 商品货号： | TS214044 |
| Organism： | Homo sapiens, human |
| Tissue： | tongue |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | squamous cell carcinoma |
| Age： | 55 years |
| Gender： | male |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Clinical Data： | male
55 years |
| Genes Expressed： | epidermal keratins; 40 kD keratin; low levels of involucrin |
| Cellular Products： | epidermal keratins; 40 kD keratin; low levels of involucrin |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | Unlike SCC-4 (ATCC CRL-1624), this line does not grow well in semi-solid medium, and does not require a feeder layer. |
| Complete Growth Medium： | A 1:1 mixture of Dulbeccos modified Eagles medium and Hams F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,14 D16S539: 12,15 D5S818: 12 D7S820: 10,11 THO1: 9,9.3 TPOX: 8 vWA: 15,17 |
| Name of Depositor： | JG Rheinwald |
| Deposited As： | Homo sapiens |
| References： | Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336 Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916 Wu YJ, Rheinwald JG. A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Cell 25: 627-635, 1981. PubMed: 6169443 |
