| 产品名称： | Bovine rhinitis B virus |
|---|---|
| 商品货号： | TS214207 |
| Classification： | Picornaviridae, Aphthovirus |
| Agent： | Bovine rhinitis B virus |
| Strain： | EC-11 |
| Common Name： | Bovine rhinovirus 2 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Derived by adaptation from primary cell culture product ATCC® VR-392™ to MDBK cells (ATCC® CCL-22™). ATCC® VR-392™ and ATCC® VR-1806™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture. Strain is 25-27 nm in diameter with characteristic rhinovirus structure. Antigenically distinct from Bovine rhinitis A virus, strain SD-1. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | MDBK cells (ATCC® CCL-22™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | SB Mohanty, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland |
| Source： | Lungs of a specific-pathogen-free Jersey calf |
| References： | Betts AO, et al. Studies on a rhinovirus (EC11) derived from a calf. II. Disease in calves. J. Comp. Pathol. 81: 41-48, 1971. PubMed: 4326296 Reed SE, et al. Studies on a rhinovirus (EC11) derived from a calf. I. Isolation in calf tracheal organ cultures and characterization of the virus. J. Comp. Pathol. 81: 33-40, 1971. PubMed: 4326295 |
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| 产品名称： | Bovine rhinitis B virus |
|---|---|
| 商品货号： | TS214207 |
| Classification： | Picornaviridae, Aphthovirus |
| Agent： | Bovine rhinitis B virus |
| Strain： | EC-11 |
| Common Name： | Bovine rhinovirus 2 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Derived by adaptation from primary cell culture product ATCC® VR-392™ to MDBK cells (ATCC® CCL-22™). ATCC® VR-392™ and ATCC® VR-1806™ have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture. Strain is 25-27 nm in diameter with characteristic rhinovirus structure. Antigenically distinct from Bovine rhinitis A virus, strain SD-1. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | MDBK cells (ATCC® CCL-22™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | SB Mohanty, Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland |
| Source： | Lungs of a specific-pathogen-free Jersey calf |
| References： | Betts AO, et al. Studies on a rhinovirus (EC11) derived from a calf. II. Disease in calves. J. Comp. Pathol. 81: 41-48, 1971. PubMed: 4326296 Reed SE, et al. Studies on a rhinovirus (EC11) derived from a calf. I. Isolation in calf tracheal organ cultures and characterization of the virus. J. Comp. Pathol. 81: 33-40, 1971. PubMed: 4326295 |
