| 产品名称： | LL20NS01 |
|---|---|
| 商品货号： | TS214424 |
| Designations： | LL20NS01 |
| Species： | Homo sapiens, human |
| Depositors： | M Van Dilla, JA Garnes |
| Origin： | skin fibroblast 811 cell line |
| Vector： | Tissue: skin fibroblast 811 cell line Chromosome: 20 DNA: genomic Digest: Complete Insert size range: 3.1 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 11000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | The #20 peak was well-isolated. One of 6 unique sequence inserts did not come from chromosomes 8, 18, 19, or 20. Of 20 probes selected for being repeat negative, 12 had inserts with repeats, 2 had inserts <500 bp, and 5/6 of the remaining unique sequence inserts hybridized to fragments of corresponding size in chromosome 20. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 354. |
| References： | . Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication |
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| 产品名称： | LL20NS01 |
|---|---|
| 商品货号： | TS214424 |
| Designations： | LL20NS01 |
| Species： | Homo sapiens, human |
| Depositors： | M Van Dilla, JA Garnes |
| Origin： | skin fibroblast 811 cell line |
| Vector： | Tissue: skin fibroblast 811 cell line Chromosome: 20 DNA: genomic Digest: Complete Insert size range: 3.1 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 11000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | The #20 peak was well-isolated. One of 6 unique sequence inserts did not come from chromosomes 8, 18, 19, or 20. Of 20 probes selected for being repeat negative, 12 had inserts with repeats, 2 had inserts <500 bp, and 5/6 of the remaining unique sequence inserts hybridized to fragments of corresponding size in chromosome 20. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 354. |
| References： | . Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986. Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319 Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320 Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712 Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744 Marvin Van Dilla, personal communication |
