| 产品名称： | Human rhinovirus 11 |
|---|---|
| 商品货号： | TS214522 |
| Classification： | Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent： | Human rhinovirus 11 |
| Strain： | 1 1-CV-15 |
| Applications： | Respiratory research |
| Biosafety Level： | 2 nonpathogenic for common laboratory animals
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | Properties of virus similar to other picornaviruses, except unstable at pH 3.0-5.0, no hemagglutinins demonstrated, non-pathogenic for common laboratory animals. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects, refractile rounding and sloughing CPE, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 H1- HeLaxa0 (ATCC CRL-1958) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 33.0°C Duration: 1 to 7 days on a rocker; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | ATCC, RM Conant, VV Hamparian |
| Special Collection： | ATCC |
| Source： | derived from existing strain |
| References： | Hamparian VV, et al. Recovery of new viruses (coryzavirus) from cases of common cold in human adults. Proc. Soc. Exp. Biol. Med. 108: 444-453, 1961. PubMed: 13904225 Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698 or colder This virus was derived by treating Mycoplasma orale contaminated ATCC VR-491 with solvent, 1,1,2-Trichlorotrifluoro-ethane. ATCC VR-491 was isolated from human throat washings. from some strains stable, human cell cultures or colder |
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| 产品名称： | Human rhinovirus 11 |
|---|---|
| 商品货号： | TS214522 |
| Classification： | Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent： | Human rhinovirus 11 |
| Strain： | 1 1-CV-15 |
| Applications： | Respiratory research |
| Biosafety Level： | 2 nonpathogenic for common laboratory animals
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | Properties of virus similar to other picornaviruses, except unstable at pH 3.0-5.0, no hemagglutinins demonstrated, non-pathogenic for common laboratory animals. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects, refractile rounding and sloughing CPE, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 H1- HeLaxa0 (ATCC CRL-1958) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 33.0°C Duration: 1 to 7 days on a rocker; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | ATCC, RM Conant, VV Hamparian |
| Special Collection： | ATCC |
| Source： | derived from existing strain |
| References： | Hamparian VV, et al. Recovery of new viruses (coryzavirus) from cases of common cold in human adults. Proc. Soc. Exp. Biol. Med. 108: 444-453, 1961. PubMed: 13904225 Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698 or colder This virus was derived by treating Mycoplasma orale contaminated ATCC VR-491 with solvent, 1,1,2-Trichlorotrifluoro-ethane. ATCC VR-491 was isolated from human throat washings. from some strains stable, human cell cultures or colder |
