| 产品名称： | Transmissible gastroenteritis virus, porcine |
|---|---|
| 商品货号： | TS214610 |
| Classification： | Coronaviridae, Coronavirus |
| Agent： | Transmissible gastroenteritis virus, porcine |
| Strain： | Miller (TC-adapted) |
| Common Name： | Transmissible gastroenteritis virus, porcine |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC. |
| Product Format： | frozen |
| Storage Conditions： | xa0Store at -70°C or colder |
| Comments： | This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC. |
| Recommended Host： | Production Host:ST cells (ATCC CRL-1746) Recommended Host: xa0ST cells (ATCC® CRL-1746x99) |
| Growth Conditions： | Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and rinse cells twice with VGM without TPCK-treated trypsin. Leave the second wash over the cells for 3 hours at 37.0°C in a humidified 5% CO2 atmosphere. Remove the second wash from the cells and then inoculate with a small volume of virus diluted with VGM supplemented with TPCK- treated trypsin (1 µg/ mL) to provide a MOI of about 0.1. Adsorb for 1 hour at 37.0°C in a humidified 5% CO2 atmosphere. End adsorption by adding VGM supplemented with 1 µg/ mL of TPCK- treated trypsin. Incubate infected culture for 3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 70-80% of the culture. |
| Name of Depositor： | LJ Saif |
| Source： | Small intestine of neonatal pig with porcine transmissible gastroenteritis. |
| References： | Bohl EH, et al. Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect. Immun. 6: 289-301, 1972. PubMed: 4629259 Bohl EH, Kumagai T. The use of cell cultures for the study of TGE virus of swine. Proc. U.S. Livestock Sanit. Assoc. 69: 343-350, 1965. |
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| 产品名称： | Transmissible gastroenteritis virus, porcine |
|---|---|
| 商品货号： | TS214610 |
| Classification： | Coronaviridae, Coronavirus |
| Agent： | Transmissible gastroenteritis virus, porcine |
| Strain： | Miller (TC-adapted) |
| Common Name： | Transmissible gastroenteritis virus, porcine |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC. |
| Product Format： | frozen |
| Storage Conditions： | xa0Store at -70°C or colder |
| Comments： | This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC. |
| Recommended Host： | Production Host:ST cells (ATCC CRL-1746) Recommended Host: xa0ST cells (ATCC® CRL-1746x99) |
| Growth Conditions： | Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and rinse cells twice with VGM without TPCK-treated trypsin. Leave the second wash over the cells for 3 hours at 37.0°C in a humidified 5% CO2 atmosphere. Remove the second wash from the cells and then inoculate with a small volume of virus diluted with VGM supplemented with TPCK- treated trypsin (1 µg/ mL) to provide a MOI of about 0.1. Adsorb for 1 hour at 37.0°C in a humidified 5% CO2 atmosphere. End adsorption by adding VGM supplemented with 1 µg/ mL of TPCK- treated trypsin. Incubate infected culture for 3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 70-80% of the culture. |
| Name of Depositor： | LJ Saif |
| Source： | Small intestine of neonatal pig with porcine transmissible gastroenteritis. |
| References： | Bohl EH, et al. Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect. Immun. 6: 289-301, 1972. PubMed: 4629259 Bohl EH, Kumagai T. The use of cell cultures for the study of TGE virus of swine. Proc. U.S. Livestock Sanit. Assoc. 69: 343-350, 1965. |
