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6F4C5
6F4C5
规格:
货期:
编号:TS214615
品牌:Testobio
产品名称: 6F4C5
商品货号: TS214615
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane.
Derivation:
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI)
Cellular Products:
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI)
Tumorigenic: Yes
Effects:
Yes, in BALB/c mice
Comments:
Animals were immunized with a galactosyl transferase rich microsomal fraction of rat myeloma cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: AM Tartakoff, Y Chicheportiche
Deposited As: mouse (B cell); mouse (myeloma)
References:

Chicheportiche Y, et al. Characterization of cytoplasmically oriented Golgi proteins with a monoclonal antibody. J. Cell Biol. 99: 2200-2210, 1984. PubMed: 6438114

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6F4C5

  • 货号: TS214615
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  • 品牌 : TESTOBIO
产品名称: 6F4C5
商品货号: TS214615
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane.
Derivation:
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI)
Cellular Products:
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI)
Tumorigenic: Yes
Effects:
Yes, in BALB/c mice
Comments:
Animals were immunized with a galactosyl transferase rich microsomal fraction of rat myeloma cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: AM Tartakoff, Y Chicheportiche
Deposited As: mouse (B cell); mouse (myeloma)
References:

Chicheportiche Y, et al. Characterization of cytoplasmically oriented Golgi proteins with a monoclonal antibody. J. Cell Biol. 99: 2200-2210, 1984. PubMed: 6438114

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