311-3D4
311-3D4
规格:
货期:
编号:TS214755
品牌:Testobio
| 产品名称： | 311-3D4 |
|---|---|
| 商品货号： | TS214755 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, but many cells attach |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene). In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN. In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells. |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Comments： | Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene). Spleen cells were fused with Sp2/0 myeloma cells. In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN. In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells. The IgG1 reactivity of the antibody with the ABL peptide (429 to 449) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Scrape off the attached cells and transfer along with the floating cells into new flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 and IgG2b |
| Antigenic Determinant： | abl oncogene peptide, synthetic |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
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| 产品名称： | 311-3D4 |
|---|---|
| 商品货号： | TS214755 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, but many cells attach |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene). In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN. In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells. |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide |
| Comments： | Animals were immunized with a synthetic polypeptide with the sequence LWEIATYGMSPYPGIDLSQVY(C) corresponding to peptides 429 to 449 of the abl oncogene (formerly 548 to 568 of the v-abl oncogene). Spleen cells were fused with Sp2/0 myeloma cells. In ELISA testing, the antibody reacts with the abl sequence. No binding activity was detected for the homologous region of FES, v-erb B, v-src, SRC, v-yes, RAF-1, v-raf, fms, v-fps, v-ros, HIR, v-mos, and SYN. In immunoblot testing a protein of approximately 40 kd is detected in ANN-1 and src transformed cells. The IgG1 reactivity of the antibody with the ABL peptide (429 to 449) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Scrape off the attached cells and transfer along with the floating cells into new flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 and IgG2b |
| Antigenic Determinant： | abl oncogene peptide, synthetic |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
