S16Y

规格:

货期:

编号:TS214756

品牌:Testobio

产品名称：	S16Y
商品货号：	TS214756
Organism：	Rattus norvegicus, rat
Tissue：	sciatic nerve
Cell Type：	Schwann cell
Product Format：	frozen
Morphology：	spindle-shaped
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	neonatal
Applications：	16Y, S16 (ATCC CRL-2941) and S42 (ATCC CRL-2942) cells should be useful for investigating the cell biology of MAG and other myelin-related components as the levels of MAG expression in the three lines are inversely related to their rates of proliferation.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The S16Y cell line arose spontaneousely from a passage of the S16 (ATCC CRL-2941), a line that was derived from a primary culture of Schwann cells and was immortalized by repetitive passaging.
Antigen Expression：	Myelin-associated glycoprotein (MAG), not expressed Galactocerebroside (GalC), not expressed P₀ glycoprotein, not expressed
Genes Expressed：	Myelin-associated glycoprotein (MAG), not expressed,Galactocerebroside (GalC), not expressed,P₀ glycoprotein, not expressed
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: The culture flasks should be treated with 0.1mL/cm^2xa0of flask surface area with 15 µg/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.xa0 Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x gxa0 for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:5 to 1:12 twice weekly is recommended. Medium renewal: Every 2 to 3 days.
Cryopreservation：	Freeze medium: Complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time：	approximately 20 hours
Name of Depositor：	RH Quarles
Year of Origin：	1989
References：	Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597 Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958 Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295

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S16Y

货号: TS214756
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产品名称：	S16Y
商品货号：	TS214756
Organism：	Rattus norvegicus, rat
Tissue：	sciatic nerve
Cell Type：	Schwann cell
Product Format：	frozen
Morphology：	spindle-shaped
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	neonatal
Applications：	16Y, S16 (ATCC CRL-2941) and S42 (ATCC CRL-2942) cells should be useful for investigating the cell biology of MAG and other myelin-related components as the levels of MAG expression in the three lines are inversely related to their rates of proliferation.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The S16Y cell line arose spontaneousely from a passage of the S16 (ATCC CRL-2941), a line that was derived from a primary culture of Schwann cells and was immortalized by repetitive passaging.
Antigen Expression：	Myelin-associated glycoprotein (MAG), not expressed Galactocerebroside (GalC), not expressed P₀ glycoprotein, not expressed
Genes Expressed：	Myelin-associated glycoprotein (MAG), not expressed,Galactocerebroside (GalC), not expressed,P₀ glycoprotein, not expressed
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: The culture flasks should be treated with 0.1mL/cm^2xa0of flask surface area with 15 µg/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.xa0 Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x gxa0 for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:5 to 1:12 twice weekly is recommended. Medium renewal: Every 2 to 3 days.
Cryopreservation：	Freeze medium: Complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time：	approximately 20 hours
Name of Depositor：	RH Quarles
Year of Origin：	1989
References：	Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597 Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958 Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295