pNO1523
pNO1523
规格:
货期:
编号:TS214905
品牌:Testobio
| 产品名称： | pNO1523 |
|---|---|
| 商品货号： | TS214905 |
| Designations： | pNO1523 |
| Depositors： | D Dean |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1999998092651370 Vector: pNO1523 (plasmid) Promoters: Promoter str Construction: pNO1517 Marker(s):ampR Construct size (kb): 5.199999809265137 Features: insert detection: rpsL+ marker(s): ampR promoter: str replicon: pMB1 |
| Applications： | vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2. A vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634 |
| Shipped： | freeze-dried |
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| 产品名称： | pNO1523 |
|---|---|
| 商品货号： | TS214905 |
| Designations： | pNO1523 |
| Depositors： | D Dean |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1999998092651370 Vector: pNO1523 (plasmid) Promoters: Promoter str Construction: pNO1517 Marker(s):ampR Construct size (kb): 5.199999809265137 Features: insert detection: rpsL+ marker(s): ampR promoter: str replicon: pMB1 |
| Applications： | vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI--5.2; EcoRI--5.2. A vector for positive selection of clones with inserts based on the dominance of the plasmid-coded rpsL+ gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Dean D. A plasmid cloning vector for the direct selection of strains carrying recombinant plasmids. Gene 15: 99-102, 1981. PubMed: 6271634 |
| Shipped： | freeze-dried |
