| 产品名称： | Epizootic hemorrhagic disease virus 1 |
|---|---|
| 商品货号： | TS214917 |
| Classification： | Reoviridae, Orbivirus, Epizootic hemorrhagic disease virus 1 |
| Agent： | Epizootic hemorrhagic disease virus 1 |
| Strain： | OV202 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Strain fully sequenced by depositor. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Loeb, University of Florida |
| Source： | Asymptomatic, farmed white-tailed deer, Gadsden County, Florida, USA. Isolated on September 22, 2015. |
| References： | Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661 Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175 |
| Cross References： | Nucleotide (GenBank) : MF688826.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP1 protein (VP1) gene, complete cds Nucleotide (GenBank) : MF688827.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP2 protein (VP2) gene, complete cds. Nucleotide (GenBank) : MF688828.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP3 protein (VP3) gene, complete cds Nucleotide (GenBank) : MF688829.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP4 protein (VP4) gene, complete cds Nucleotide (GenBank) : MF688830.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP5 protein (VP5) gene, complete cds Nucleotide (GenBank) : MF688831.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP6 protein (VP6) gene, complete cds Nucleotide (GenBank) : MF688832.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP7 protein (VP7) gene, complete cds Nucleotide (GenBank) : MF688833.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS1 protein (NS1) gene, complete cds Nucleotide (GenBank) : MF688834.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS2 protein (NS2) gene, complete cds Nucleotide (GenBank) : MF688835.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS3 protein (NS3) gene, complete cds |
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| 产品名称： | Epizootic hemorrhagic disease virus 1 |
|---|---|
| 商品货号： | TS214917 |
| Classification： | Reoviridae, Orbivirus, Epizootic hemorrhagic disease virus 1 |
| Agent： | Epizootic hemorrhagic disease virus 1 |
| Strain： | OV202 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： | |
| Comments： | Strain fully sequenced by depositor. |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | J Loeb, University of Florida |
| Source： | Asymptomatic, farmed white-tailed deer, Gadsden County, Florida, USA. Isolated on September 22, 2015. |
| References： | Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661 Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175 |
| Cross References： | Nucleotide (GenBank) : MF688826.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP1 protein (VP1) gene, complete cds Nucleotide (GenBank) : MF688827.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP2 protein (VP2) gene, complete cds. Nucleotide (GenBank) : MF688828.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP3 protein (VP3) gene, complete cds Nucleotide (GenBank) : MF688829.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP4 protein (VP4) gene, complete cds Nucleotide (GenBank) : MF688830.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP5 protein (VP5) gene, complete cds Nucleotide (GenBank) : MF688831.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP6 protein (VP6) gene, complete cds Nucleotide (GenBank) : MF688832.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP7 protein (VP7) gene, complete cds Nucleotide (GenBank) : MF688833.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS1 protein (NS1) gene, complete cds Nucleotide (GenBank) : MF688834.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS2 protein (NS2) gene, complete cds Nucleotide (GenBank) : MF688835.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS3 protein (NS3) gene, complete cds |
