| 产品名称： | Pseudomonas aeruginosa GFP |
|---|---|
| 商品货号： | TS214932 |
| Verified By： | Whole-genome Sequencing |
| Application： | Fluorescence detection Fluorescence imaging FACS Cell infection assays In vivo localization Pathogen-host interactions assays |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Type Strain： | no |
| Antibiotic Resistance： | Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin. |
| Comments： | This clone was derived from ATCC® 10145™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling. Vector: pUCP18-MCSgfpmut3 Vector size: 5.492kb Vector type: plasmid Marker: bla (ampR) Features: GFPmut3: fluorescent maker under the control of Plac promoter Origin of replication: ori from pRO1600 Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI Double restriction sites: HindIII, PstI, SalI, XbaI |
| Medium： | ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | M Barbier |
| Chain of Custody： | Modified by ATCC, Dr. M. Barbier <-- ATCC <-- F Kavanagh <-- Merck Sharp & Dohme |
| References： | Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster. Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227 Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970. Hugh R, Leifson E. The proposed Neotype Strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900. Int. Bull. Bacteriol. Nomencl. Taxon 14: 69-84, 1964. |
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| 产品名称： | Pseudomonas aeruginosa GFP |
|---|---|
| 商品货号： | TS214932 |
| Verified By： | Whole-genome Sequencing |
| Application： | Fluorescence detection Fluorescence imaging FACS Cell infection assays In vivo localization Pathogen-host interactions assays |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Type Strain： | no |
| Antibiotic Resistance： | Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin. |
| Comments： | This clone was derived from ATCC® 10145™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling. Vector: pUCP18-MCSgfpmut3 Vector size: 5.492kb Vector type: plasmid Marker: bla (ampR) Features: GFPmut3: fluorescent maker under the control of Plac promoter Origin of replication: ori from pRO1600 Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI Double restriction sites: HindIII, PstI, SalI, XbaI |
| Medium： | ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | M Barbier |
| Chain of Custody： | Modified by ATCC, Dr. M. Barbier <-- ATCC <-- F Kavanagh <-- Merck Sharp & Dohme |
| References： | Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster. Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227 Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970. Hugh R, Leifson E. The proposed Neotype Strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900. Int. Bull. Bacteriol. Nomencl. Taxon 14: 69-84, 1964. |
