| 产品名称： | Staphylococcus aureus Rosenbach |
|---|---|
| 商品货号： | TS215125 |
| Strain Designations： | M10/0061 |
| Application： | Drug discovery |
| Isolation： | Isolated from an 85-year old male in-patient in a regional hospital in southeastern Ireland, May 2010. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | pvl negative |
| Genome Sequenced Strain： | Yes |
| Type Strain： | no |
| Antibiotic Resistance： | Methicillin resistant |
| Comments： | Whole-genome sequencing of this isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). Presence of mecC gene confirmed by PCR The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. Note: The isolate was identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay, which is incorrect! MRSA SCCmec: Type XI spa type Ridom: t843 spa type Kreiswirth: Zr82MOMOOKM pvl gene amplification: Negative Phenotypic and genotypic information for ATCC MRSA strains, such as SCCmec, PFGE, or spa typing, is provided for informational purposes only. This information was compiled from testing performed by one or more sources, including ATCC, strain depositor, published data, or contracted research. |
| Medium： | ATCC® Medium 3: Nutrient agar or nutrient broth |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | D Coleman |
| References： | Shore AC, et al. Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 55(8): 3765-3773, 2011. PubMed: 21636525 Cuny C, et al. Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany. PLoS One 6(9): e24360, 2011. PubMed: 21931689 Monecke S, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6(4): e17936, 2011. PubMed: 21494333 |
| Cross References： | Nucleotide (GenBank) : FR823292.1 Staphylococcus aureus Staphylococcal Cassette Chromosome mec element XI (SCCmec XI), strain M10/0061 |
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| 产品名称： | Staphylococcus aureus Rosenbach |
|---|---|
| 商品货号： | TS215125 |
| Strain Designations： | M10/0061 |
| Application： | Drug discovery |
| Isolation： | Isolated from an 85-year old male in-patient in a regional hospital in southeastern Ireland, May 2010. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | pvl negative |
| Genome Sequenced Strain： | Yes |
| Type Strain： | no |
| Antibiotic Resistance： | Methicillin resistant |
| Comments： | Whole-genome sequencing of this isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). Presence of mecC gene confirmed by PCR The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. Note: The isolate was identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay, which is incorrect! MRSA SCCmec: Type XI spa type Ridom: t843 spa type Kreiswirth: Zr82MOMOOKM pvl gene amplification: Negative Phenotypic and genotypic information for ATCC MRSA strains, such as SCCmec, PFGE, or spa typing, is provided for informational purposes only. This information was compiled from testing performed by one or more sources, including ATCC, strain depositor, published data, or contracted research. |
| Medium： | ATCC® Medium 3: Nutrient agar or nutrient broth |
| Growth Conditions： | Temperature: 37°C Atmosphere: Aerobic |
| Name of Depositor： | D Coleman |
| References： | Shore AC, et al. Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 55(8): 3765-3773, 2011. PubMed: 21636525 Cuny C, et al. Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany. PLoS One 6(9): e24360, 2011. PubMed: 21931689 Monecke S, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6(4): e17936, 2011. PubMed: 21494333 |
| Cross References： | Nucleotide (GenBank) : FR823292.1 Staphylococcus aureus Staphylococcal Cassette Chromosome mec element XI (SCCmec XI), strain M10/0061 |
