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CMT-93
CMT-93
规格:
货期:
编号:TS215173
品牌:Testobio
产品名称: CMT-93
商品货号: TS215173
Organism: Mus musculus, mouse
Tissue: rectum
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: polyploid carcinoma
Age: 19 months
Gender: male
Strain: C57BL/icrf
Storage Conditions: liquid nitrogen vapor phase
Images:
Clinical Data:
male
Antigen Expression:
H-2b
Tumorigenic: Yes
Effects:
Yes, in nude mice
Comments: Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0
  5. Add appropriate aliquots of the cell supension to new culture vessels.xa0
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
Name of Depositor: LM Franks
Deposited As: Mus musculus
References:

Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978. PubMed: 722371

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CMT-93

  • 货号: TS215173
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: CMT-93
商品货号: TS215173
Organism: Mus musculus, mouse
Tissue: rectum
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: polyploid carcinoma
Age: 19 months
Gender: male
Strain: C57BL/icrf
Storage Conditions: liquid nitrogen vapor phase
Images:
Clinical Data:
male
Antigen Expression:
H-2b
Tumorigenic: Yes
Effects:
Yes, in nude mice
Comments: Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0
  5. Add appropriate aliquots of the cell supension to new culture vessels.xa0
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37°C
Name of Depositor: LM Franks
Deposited As: Mus musculus
References:

Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978. PubMed: 722371

合作单位: