Phoenix-AMPHO

规格:

货期:

编号:TS215175

品牌:Testobio

产品名称：	Phoenix-AMPHO
商品货号：	TS215175
Organism：	Homo sapiens, human
Tissue：	kidney
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Adeno and SV-40 viral DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo
Applications：	Phoenix is a second-generation retrovirus producerxa0cell linexa0for the generation of helper-free ecotropic and amphotropic retroviruses. The unique feature of this cell line is that it is highly transfectable with either calcium phosphate mediated transfection or lipid-based transfection protocols-- up to 50% or higher of cells can be transiently transfected. This cell line is capable of carrying episomes for long-term stable production of retrovirus (LZRS).
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	HEK 293T/17 cells were transformed with adenovirus E1a carrying a temperature sensitive T antigen co-selected with neomycin. Transformation was brought about by the insertion of approximately 4.5 kilobases of viral genome into human chromosome 19.
Comments：	Phoenix Amphotropic (AMPHO) cell line expresses amphotropic envelope protein. CD8 expression is a direct reflection of intracellular gag-pol and the stability of the producer cell populations ability to produce gag-pol can be readily monitored by flow cytometry. Phoenix-Ampho have been extensively tested for helper virus production and established as being helper-virus free. xa0
Complete Growth Medium：	The base medium for this cell line is Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC 30-2002). To make the complete growth medium, add the following components to the base medium: 10% Fetal Bovine Serum (heat inactivated) (ATCC 30-2020), 2mM L-glutamine (ATCC 30-2214), 1% Penicillin/Streptomycin.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Note:xa0xa0Culture to ≤80% confluence to prevent the cells from sloughing Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:8 is recommended Medium Renewal: Every 2 to 3 days xa0 xa0
Cryopreservation：	Freeze medium:xa0 complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Cells per Vial：	4.5 x 10⁶ cells per vial
STR Profile：	Amelogenin: X CSF1PO: 11,12 D13S317: 11,12,13,14 D16S539: 9,13 D5S818: 8,10 D7S820: 11 TH01: 7,9.3 TPOX: 11 vWA: 16,18,19
Population Doubling Time：	16 hours
Name of Depositor：	G Nolan
Year of Origin：	1996
References：	Swift S et al. Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293T Cell-Based Systems. Current Protocols in Immunology,Unit 10.17C, 2001 Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960 Nolan Laboratory Retroviral Systems. Phoenix helper-free retrovirus producer lines, https://web.stanford.edu/group/nolan/_OldWebsite/retroviral_systems/retsys.html

首页 > 产品中心 > 微生物培养 > 菌株 > null > Phoenix-AMPHO

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

Phoenix-AMPHO

货号: TS215175
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	Phoenix-AMPHO
商品货号：	TS215175
Organism：	Homo sapiens, human
Tissue：	kidney
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Adeno and SV-40 viral DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo
Applications：	Phoenix is a second-generation retrovirus producerxa0cell linexa0for the generation of helper-free ecotropic and amphotropic retroviruses. The unique feature of this cell line is that it is highly transfectable with either calcium phosphate mediated transfection or lipid-based transfection protocols-- up to 50% or higher of cells can be transiently transfected. This cell line is capable of carrying episomes for long-term stable production of retrovirus (LZRS).
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	HEK 293T/17 cells were transformed with adenovirus E1a carrying a temperature sensitive T antigen co-selected with neomycin. Transformation was brought about by the insertion of approximately 4.5 kilobases of viral genome into human chromosome 19.
Comments：	Phoenix Amphotropic (AMPHO) cell line expresses amphotropic envelope protein. CD8 expression is a direct reflection of intracellular gag-pol and the stability of the producer cell populations ability to produce gag-pol can be readily monitored by flow cytometry. Phoenix-Ampho have been extensively tested for helper virus production and established as being helper-virus free. xa0
Complete Growth Medium：	The base medium for this cell line is Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC 30-2002). To make the complete growth medium, add the following components to the base medium: 10% Fetal Bovine Serum (heat inactivated) (ATCC 30-2020), 2mM L-glutamine (ATCC 30-2214), 1% Penicillin/Streptomycin.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Note:xa0xa0Culture to ≤80% confluence to prevent the cells from sloughing Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:8 is recommended Medium Renewal: Every 2 to 3 days xa0 xa0
Cryopreservation：	Freeze medium:xa0 complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Cells per Vial：	4.5 x 10⁶ cells per vial
STR Profile：	Amelogenin: X CSF1PO: 11,12 D13S317: 11,12,13,14 D16S539: 9,13 D5S818: 8,10 D7S820: 11 TH01: 7,9.3 TPOX: 11 vWA: 16,18,19
Population Doubling Time：	16 hours
Name of Depositor：	G Nolan
Year of Origin：	1996
References：	Swift S et al. Rapid Production of Retroviruses for Efficient Gene Delivery to Mammalian Cells Using 293T Cell-Based Systems. Current Protocols in Immunology,Unit 10.17C, 2001 Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960 Nolan Laboratory Retroviral Systems. Phoenix helper-free retrovirus producer lines, https://web.stanford.edu/group/nolan/_OldWebsite/retroviral_systems/retsys.html