Polynucleobacter difficilis
Polynucleobacter difficilis
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编号:TS244062
品牌:Testobio
| 产品名称： | Polynucleobacter difficilis |
|---|---|
| 商品货号： | TS244062 |
| <strong>Name </strong>： | Polynucleobacter difficilis Hahn et al. 2012 |
| <strong>DSM No. </strong>： | 22349, Type strain dsm-22349 dsm22349 dsm 22349 Polynucleobacter difficilis |
| Strain designation ： | AM-8B5 |
| Other collection no. <br />or WDCM no. ： | CIP 110078 |
| Isolated from ： | freshwater taken from a depth of 60 m close to the bottom of a eutrophic lake |
| Country ： | Armenia Lake Sevan (40° 29 35.50\ N 45° 11 31.34\ E) |
| Date of sampling ： | before 09.03.2009 |
| <strong>Nagoya Protocol Restrictions </strong>： | There are NO known Nagoya Protocol restrictions for this strain. |
| History ： | |
| Genbank accession numbers ： | 16S rRNA gene, IGS and 23S rRNA gene: FM208181 |
| Cultivation conditions ： | Medium 830 diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each, 25°C Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation and delivery period several weeks Incubation time: more than 14 days Complete DSMZ Media List |
| Summary and <br />additional information ： | Type strain. Taxonomy/description (16649). Sequence accession no. 16S rRNA gene, IGS and 23S rRNA gene: FM208181. (Medium 830 diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each, 25°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation and delivery period several weeks. |
| For detailed information ： | BacDive - The Bacterial Diversity Metadatabase |
| Literature ： | 16649 |
| Risk group ： | 1 (classification according to German TRBA) |
| Other cultures ： | All DSMZ cultures of the species |
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| 产品名称： | Polynucleobacter difficilis |
|---|---|
| 商品货号： | TS244062 |
| <strong>Name </strong>： | Polynucleobacter difficilis Hahn et al. 2012 |
| <strong>DSM No. </strong>： | 22349, Type strain dsm-22349 dsm22349 dsm 22349 Polynucleobacter difficilis |
| Strain designation ： | AM-8B5 |
| Other collection no. <br />or WDCM no. ： | CIP 110078 |
| Isolated from ： | freshwater taken from a depth of 60 m close to the bottom of a eutrophic lake |
| Country ： | Armenia Lake Sevan (40° 29 35.50\ N 45° 11 31.34\ E) |
| Date of sampling ： | before 09.03.2009 |
| <strong>Nagoya Protocol Restrictions </strong>： | There are NO known Nagoya Protocol restrictions for this strain. |
| History ： | |
| Genbank accession numbers ： | 16S rRNA gene, IGS and 23S rRNA gene: FM208181 |
| Cultivation conditions ： | Medium 830 diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each, 25°C Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation and delivery period several weeks Incubation time: more than 14 days Complete DSMZ Media List |
| Summary and <br />additional information ： | Type strain. Taxonomy/description (16649). Sequence accession no. 16S rRNA gene, IGS and 23S rRNA gene: FM208181. (Medium 830 diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each, 25°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate. Incubation and delivery period several weeks. |
| For detailed information ： | BacDive - The Bacterial Diversity Metadatabase |
| Literature ： | 16649 |
| Risk group ： | 1 (classification according to German TRBA) |
| Other cultures ： | All DSMZ cultures of the species |
