HL-60
HL-60
规格:
货期:
编号:TS258759
品牌:Testobio
| 产品名称： | HL-60 |
|---|---|
| 商品货号： | TS258759 |
| <strong>Cell line </strong>： | HL-60 |
| DSMZ no. ： | TS258759 |
| Species ： | confirmed originally as human with IEF of MDH, NP and later by STR fingerprinting |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described in the literature to be responsive to DMSO, phorbol ester TPA and other reagents (e.g. expression of TNF alpha) and to carry amplified MYC gene; present cells are apparently tetraploid derivates of hypodiploid original where MYC was amplified in dmin (instead of hsr). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14548, 14437, 14438, 14443, 15215, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at about 1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml, split 1:2 to 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | maximal density of 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with hypodiploid sideline and 1.5% polyploidy - 82-88<4n>XX, -X, -X, -8, -8, -16, -17, -17, +18, +22, +2mar, ins(1;8)(p?31;q24hsr)x2, der(5)t(5;17)(q11;q11)x2, add(6)(q27)x2, der(9)del(9)(p13)t(9;14)(q?22;q?22)x2, der(14)t(9;14)(q?22;q?22)x2, der(16)t(16;17)(q22;q22)x1-2, add(18)(q21) - sideline with: -2, -5, -15, del(11)(q23.1q23.2) - c-myc amplicons present in der(1) and in both markers |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 - , HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
HL-60
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | HL-60 |
|---|---|
| 商品货号： | TS258759 |
| <strong>Cell line </strong>： | HL-60 |
| DSMZ no. ： | TS258759 |
| Species ： | confirmed originally as human with IEF of MDH, NP and later by STR fingerprinting |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976; cells can be used for induction of differentiation studies; described in the literature to be responsive to DMSO, phorbol ester TPA and other reagents (e.g. expression of TNF alpha) and to carry amplified MYC gene; present cells are apparently tetraploid derivates of hypodiploid original where MYC was amplified in dmin (instead of hsr). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14548, 14437, 14438, 14443, 15215, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells in suspension; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at about 1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml, split 1:2 to 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | maximal density of 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with hypodiploid sideline and 1.5% polyploidy - 82-88<4n>XX, -X, -X, -8, -8, -16, -17, -17, +18, +22, +2mar, ins(1;8)(p?31;q24hsr)x2, der(5)t(5;17)(q11;q11)x2, add(6)(q27)x2, der(9)del(9)(p13)t(9;14)(q?22;q?22)x2, der(14)t(9;14)(q?22;q?22)x2, der(16)t(16;17)(q22;q22)x1-2, add(18)(q21) - sideline with: -2, -5, -15, del(11)(q23.1q23.2) - c-myc amplicons present in der(1) and in both markers |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 - , HTLV-I/II -, MLV -, SMRV - |
