X63AG8.653
X63AG8.653
规格:
货期:
编号:TS258839
品牌:Testobio
| 产品名称： | X63AG8.653 |
|---|---|
| 商品货号： | TS258839 |
| <strong>Cell line </strong>： | X63AG8.653 |
| DSMZ no. ： | TS258839 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | myeloma |
| Origin ： | X63AG8.653 is a non-secreting clone derived from P3X63AG8 (a Balb/c myeloma) and is recommended as fusion parent for production of hybridomas |
| Reference(s) ： | 14655 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round, large cells in suspension; image; image; image |
| Medium ： | RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.1-1.0 x 106 cells/ml; optimal split ratio of 1:10 to 1:20 every 48 hours; seed out at about 1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-50 hours |
| Harvest ： | maximal density of ca. 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
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周雪
13372109301
| 产品名称： | X63AG8.653 |
|---|---|
| 商品货号： | TS258839 |
| <strong>Cell line </strong>： | X63AG8.653 |
| DSMZ no. ： | TS258839 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | myeloma |
| Origin ： | X63AG8.653 is a non-secreting clone derived from P3X63AG8 (a Balb/c myeloma) and is recommended as fusion parent for production of hybridomas |
| Reference(s) ： | 14655 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round, large cells in suspension; image; image; image |
| Medium ： | RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.1-1.0 x 106 cells/ml; optimal split ratio of 1:10 to 1:20 every 48 hours; seed out at about 1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-50 hours |
| Harvest ： | maximal density of ca. 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
