A-498
A-498
规格:
货期:
编号:TS258860
品牌:Testobio
| 产品名称： | A-498 |
|---|---|
| 商品货号： | TS258860 |
| <strong>Cell line </strong>： | A-498 |
| DSMZ no. ： | TS258860 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | kidney carcinoma |
| Origin ： | established from the kidney carcinoma of a 52-year-old man in 1973 |
| Reference(s) ： | 14556, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, adherent cells growing as monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | split confluent cultures 1:3 to 1:5 every 3-5 days using trypsin/EDTA; seed out at ca. 1.0 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | > 60 hours |
| Harvest ： | about 2-3 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 7% polyploidy - 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9, -11, -14, +17, -18, -20, +21, +3mar, dm/r (31%) - der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q) - sideline with der(3)t(3;3)(q27;p14) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | A-498 |
|---|---|
| 商品货号： | TS258860 |
| <strong>Cell line </strong>： | A-498 |
| DSMZ no. ： | TS258860 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | kidney carcinoma |
| Origin ： | established from the kidney carcinoma of a 52-year-old man in 1973 |
| Reference(s) ： | 14556, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, adherent cells growing as monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS |
| Subculture ： | split confluent cultures 1:3 to 1:5 every 3-5 days using trypsin/EDTA; seed out at ca. 1.0 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | > 60 hours |
| Harvest ： | about 2-3 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 7% polyploidy - 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9, -11, -14, +17, -18, -20, +21, +3mar, dm/r (31%) - der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q) - sideline with der(3)t(3;3)(q27;p14) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
