规格:

货期:

编号:TS258863

品牌:Testobio

产品名称：	R1
商品货号：	TS258863
<strong>Cell line </strong>：	R1
DSMZ no. ：	TS258863
Species ：	Oncorhynchus tshawytscha origin determined by COI DNA barcoding
Cell type ：	liver
Origin ：	established from the liver of a rainbow trout; however, COI DNA barcoding revealed R1 as originating from a Chinook salmon (Oncorhynchus tshawytscha) and not from rainbow trout (Oncorhynchus mykiss) as described
Reference(s) ：	14350
Biosafety level ：	1
Permissions and restrictions ：	A
<strong> </strong>：	DSMZ Scientific Data:
Morphology：	adherent, fibroblastoid cells growing as monolayer
Medium ：	90% Medium 199 (25 mM HEPES) with Earles salts with L-glutamine + 10% h.i. FBS
Subculture ：	cell growth is extremely slow; split confluent culture 1:2 every 7 days using TNE (50 mM Tris, 0.1 M NaCl, 5 mM EDTA, pH 7.5) at +4 °C for 10 min; culture at room temperature (about 20 °C) without CO₂ (keep lid of flask closed); cells can be stored temporarily (up to 1 week) at 4 °C; seed out at ca. 0.1 x 10⁶ cells/ml
Incubation ：	at room temperature (about 20 °C) without CO₂
Doubling time ：	about 160 hours
Harvest ：	about 5 x 10⁶ cells/175 cm² - saturation density of ca. 10 x 10⁶ cells/175 cm²
Storage ：	frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma ：	contamination was eliminated by the depositor with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses ：	PCR: SMRV -

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R1

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产品名称：	R1
商品货号：	TS258863
<strong>Cell line </strong>：	R1
DSMZ no. ：	TS258863
Species ：	Oncorhynchus tshawytscha origin determined by COI DNA barcoding
Cell type ：	liver
Origin ：	established from the liver of a rainbow trout; however, COI DNA barcoding revealed R1 as originating from a Chinook salmon (Oncorhynchus tshawytscha) and not from rainbow trout (Oncorhynchus mykiss) as described
Reference(s) ：	14350
Biosafety level ：	1
Permissions and restrictions ：	A
<strong> </strong>：	DSMZ Scientific Data:
Morphology：	adherent, fibroblastoid cells growing as monolayer
Medium ：	90% Medium 199 (25 mM HEPES) with Earles salts with L-glutamine + 10% h.i. FBS
Subculture ：	cell growth is extremely slow; split confluent culture 1:2 every 7 days using TNE (50 mM Tris, 0.1 M NaCl, 5 mM EDTA, pH 7.5) at +4 °C for 10 min; culture at room temperature (about 20 °C) without CO₂ (keep lid of flask closed); cells can be stored temporarily (up to 1 week) at 4 °C; seed out at ca. 0.1 x 10⁶ cells/ml
Incubation ：	at room temperature (about 20 °C) without CO₂
Doubling time ：	about 160 hours
Harvest ：	about 5 x 10⁶ cells/175 cm² - saturation density of ca. 10 x 10⁶ cells/175 cm²
Storage ：	frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma ：	contamination was eliminated by the depositor with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses ：	PCR: SMRV -