NIH-3T3
NIH-3T3
规格:
货期:
编号:TS258872
品牌:Testobio
| 产品名称： | NIH-3T3 |
|---|---|
| 商品货号： | TS258872 |
| <strong>Cell line </strong>： | NIH-3T3 |
| DSMZ no. ： | TS258872 |
| Species ： | originally confirmed as mouse with IEF of AST, G6PD, MDH, NP, by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | embryo fibroblasts |
| Origin ： | contact-inhibited NIH Swiss mouse embryo; used in DNA transfection assays |
| Reference(s) ： | 14343, 14440, 14637 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid, adherent cells growing as monolayer; image; image; image; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS (or NCS) |
| Subculture ： | seed out at 1-2 x 106 cells/175 cm2 flask; do not allow culture to become completely confluent; split culture at 1:10 every 4 to 6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype with 3% polyploidy - 68(58-73)<3n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
NIH-3T3
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | NIH-3T3 |
|---|---|
| 商品货号： | TS258872 |
| <strong>Cell line </strong>： | NIH-3T3 |
| DSMZ no. ： | TS258872 |
| Species ： | originally confirmed as mouse with IEF of AST, G6PD, MDH, NP, by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | embryo fibroblasts |
| Origin ： | contact-inhibited NIH Swiss mouse embryo; used in DNA transfection assays |
| Reference(s) ： | 14343, 14440, 14637 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid, adherent cells growing as monolayer; image; image; image; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS (or NCS) |
| Subculture ： | seed out at 1-2 x 106 cells/175 cm2 flask; do not allow culture to become completely confluent; split culture at 1:10 every 4 to 6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 20 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype with 3% polyploidy - 68(58-73)<3n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
