COS-1
COS-1
规格:
货期:
编号:TS258884
品牌:Testobio
| 产品名称： | COS-1 |
|---|---|
| 商品货号： | TS258884 |
| <strong>Cell line </strong>： | COS-1 |
| DSMZ no. ： | TS258884 |
| Species ： | Chlorocebus aethiops (Cercopithecus aethiops) origin confirmed by COI DNA barcoding |
| Cell type ： | kidney |
| Origin ： | established from CV-1 Simian cells (cercopithecus aethiops) which were transformed by an origin-defective mutant of SV-40; cells are host cells for the propagation of pure populations of recombinant SV virus; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14568 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line was established by transfection with a mutated simian virus 40 (SV40) exhibiting a 6 bp deletion in the replication origin. This renders the virus incompetent for replication and the cell line can thus be handled under biosafety level 1. |
| Permissions and restrictions ： | A, D, E |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like, adherent monolayers; image; image; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2 in 15 ml medium; split confluent cultures 1:3 to 1:10 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile (identical to COS-7, DSMZ ACC 60) |
| Cytogenetics ： | hypotetraploid simian karyotype with 4% polyploidy - 102(95-108)<4n> - structural rearrangements present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HCV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV -, SMRV - |
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周雪
13372109301
| 产品名称： | COS-1 |
|---|---|
| 商品货号： | TS258884 |
| <strong>Cell line </strong>： | COS-1 |
| DSMZ no. ： | TS258884 |
| Species ： | Chlorocebus aethiops (Cercopithecus aethiops) origin confirmed by COI DNA barcoding |
| Cell type ： | kidney |
| Origin ： | established from CV-1 Simian cells (cercopithecus aethiops) which were transformed by an origin-defective mutant of SV-40; cells are host cells for the propagation of pure populations of recombinant SV virus; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14568 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | The cell line was established by transfection with a mutated simian virus 40 (SV40) exhibiting a 6 bp deletion in the replication origin. This renders the virus incompetent for replication and the cell line can thus be handled under biosafety level 1. |
| Permissions and restrictions ： | A, D, E |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like, adherent monolayers; image; image; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2 in 15 ml medium; split confluent cultures 1:3 to 1:10 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile (identical to COS-7, DSMZ ACC 60) |
| Cytogenetics ： | hypotetraploid simian karyotype with 4% polyploidy - 102(95-108)<4n> - structural rearrangements present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HCV -, HBV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV -, SMRV - |
