EHEB
EHEB
规格:
货期:
编号:TS258894
品牌:Testobio
| 产品名称： | EHEB |
|---|---|
| 商品货号： | TS258894 |
| <strong>Cell line </strong>： | EHEB |
| DSMZ no. ： | TS258894 |
| Species ： | confirmed originally as human with IEF of MDH, NP later by STR typing |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established from the peripheral blood of a 69-year-old woman with B-CLL (chronic lymphocytic leukemia) prior treatment by EBV-transformation in 1988; cell line may represent rather a B-lymphoblastoid cell line than a bona fide B-CLL cell line |
| Reference(s) ： | 14916 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid, round to polygonal, single or clustered cells in suspension; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; cells may grow slowly initially; use at first 24-well plates and 20% FBS; during growth phase split at ca. 1:2 every 2-3 days and maintain at 0.1-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 1.6% polyploidy - 45(42-46)<2n>X, -X - no structural rearrangements detected |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | EHEB |
|---|---|
| 商品货号： | TS258894 |
| <strong>Cell line </strong>： | EHEB |
| DSMZ no. ： | TS258894 |
| Species ： | confirmed originally as human with IEF of MDH, NP later by STR typing |
| Cell type ： | chronic B cell leukemia |
| Origin ： | established from the peripheral blood of a 69-year-old woman with B-CLL (chronic lymphocytic leukemia) prior treatment by EBV-transformation in 1988; cell line may represent rather a B-lymphoblastoid cell line than a bona fide B-CLL cell line |
| Reference(s) ： | 14916 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid, round to polygonal, single or clustered cells in suspension; image; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; cells may grow slowly initially; use at first 24-well plates and 20% FBS; during growth phase split at ca. 1:2 every 2-3 days and maintain at 0.1-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cyIgM -, cykappa +, cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 1.6% polyploidy - 45(42-46)<2n>X, -X - no structural rearrangements detected |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
