RPMI-7951
RPMI-7951
规格:
货期:
编号:TS258897
品牌:Testobio
| 产品名称： | RPMI-7951 |
|---|---|
| 商品货号： | TS258897 |
| <strong>Cell line </strong>： | RPMI-7951 |
| DSMZ no. ： | TS258897 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | malignant melanoma |
| Origin ： | established from an involved lymph node of an 18-year-old Caucasian woman with malignant melanoma in 1971 |
| Reference(s) ： | 14872 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:10 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60 hours |
| Harvest ： | about 10-12 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 22% polyploidy - 54(50-60)<2n>X, -X, +1, +2, +6, +7, +8, +20, +20, +3mar, der(8)t(7;8)(p11;p12), der(9)t(9;11)(p21;q11), add(18)(q23;HSR) - shares t(9;11) with IPC-298 defining a new recurrent translocation in malignant melanoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
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周雪
13372109301
| 产品名称： | RPMI-7951 |
|---|---|
| 商品货号： | TS258897 |
| <strong>Cell line </strong>： | RPMI-7951 |
| DSMZ no. ： | TS258897 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | malignant melanoma |
| Origin ： | established from an involved lymph node of an 18-year-old Caucasian woman with malignant melanoma in 1971 |
| Reference(s) ： | 14872 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:10 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60 hours |
| Harvest ： | about 10-12 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 22% polyploidy - 54(50-60)<2n>X, -X, +1, +2, +6, +7, +8, +20, +20, +3mar, der(8)t(7;8)(p11;p12), der(9)t(9;11)(p21;q11), add(18)(q23;HSR) - shares t(9;11) with IPC-298 defining a new recurrent translocation in malignant melanoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |
