| 产品名称: | MB-020 |
|---|---|
| 商品货号: | TS258912 |
| <strong>Cell line </strong>: | MB-020 |
| DSMZ no. : | TS258912 |
| Species : | Mamestra brassicae origin confirmed by COI DNA barcoding |
| Cell type : | larval hemocytes |
| Origin : | established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (cabbage moth; Kohleule) |
| Reference(s) : | 14712 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | polymorphic round cells, partly adherent |
| Medium : | SF-900 Insect-X Press (protein-free medium) (no h.i. FBS) |
| Subculture : | cells are difficult to culture! split confluent cultures 1:2 every day (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20 °C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4 °C for up to one week without loss of viability |
| Incubation : | at 20 °C without CO2 (at room temperature; keep lid of flask closed) |
| Harvest : | about 5-10 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses : | PCR: SMRV - |
| 产品名称: | MB-020 |
|---|---|
| 商品货号: | TS258912 |
| <strong>Cell line </strong>: | MB-020 |
| DSMZ no. : | TS258912 |
| Species : | Mamestra brassicae origin confirmed by COI DNA barcoding |
| Cell type : | larval hemocytes |
| Origin : | established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (cabbage moth; Kohleule) |
| Reference(s) : | 14712 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | polymorphic round cells, partly adherent |
| Medium : | SF-900 Insect-X Press (protein-free medium) (no h.i. FBS) |
| Subculture : | cells are difficult to culture! split confluent cultures 1:2 every day (cells grow partly adherent, but trypsin is not necessary); maintain at room temperature (about 20 °C) without CO2 exposure (keep lid of flask closed); confluent cultures can be stored at 4 °C for up to one week without loss of viability |
| Incubation : | at 20 °C without CO2 (at room temperature; keep lid of flask closed) |
| Harvest : | about 5-10 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses : | PCR: SMRV - |