| 产品名称: | 72A1 |
|---|---|
| 商品货号: | TS258928 |
| <strong>Cell line </strong>: | 72A1 |
| DSMZ no. : | TS258928 |
| Species : | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type : | hybridoma (anti-EBV) |
| Origin : | established by fusion of the P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal antibody (IgG1) that reacts with a viral envelope glycoprotein (350/220 kD) and that neutralizes EBV |
| Reference(s) : | 14612 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single cells in suspension; image; image |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | maintain at 0.1-1.0 x 106 cells/ml; split every 2-3 days at 1:3 to 1:5 |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | 1.0-1.5 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |
| 产品名称: | 72A1 |
|---|---|
| 商品货号: | TS258928 |
| <strong>Cell line </strong>: | 72A1 |
| DSMZ no. : | TS258928 |
| Species : | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type : | hybridoma (anti-EBV) |
| Origin : | established by fusion of the P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal antibody (IgG1) that reacts with a viral envelope glycoprotein (350/220 kD) and that neutralizes EBV |
| Reference(s) : | 14612 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single cells in suspension; image; image |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | maintain at 0.1-1.0 x 106 cells/ml; split every 2-3 days at 1:3 to 1:5 |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | 1.0-1.5 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |