WERI-RB-1
WERI-RB-1
规格:
货期:
编号:TS258946
品牌:Testobio
| 产品名称： | WERI-RB-1 |
|---|---|
| 商品货号： | TS258946 |
| <strong>Cell line </strong>： | WERI-RB-1 |
| DSMZ no. ： | TS258946 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | retinoblastoma |
| Origin ： | established from retinoblastoma tissue of a 1-year-old Caucasian girl in 1974 |
| Reference(s) ： | 14757, 14758 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing as grape-like clusters of round cells in suspension; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | optimal split ratio of 1:2 to 1:3 once a week; maintain at ca. 0.3-0.5 x 106 cells/ml; maximal cell density of about 1.5 x 106 cells/ml; seed out at ca. 0.3-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 49 hours |
| Harvest ： | cell harvest of about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 3.9% polyploidy - 46(41-48)<2n>XX, +6, -10, -10, -14, -22, +3mar, add(3)(q25), add(3)(q25), add(4)(p15), add(5)(q35), i(6q), del(7)(p21), add(9)(q33), der(13)x2, add(16)(q23), add(16)(q23), i(17q), add(19)(q13) - apparently (uniparental?) disomic rearrangement of ch 13 - corresponds to reported karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | WERI-RB-1 |
|---|---|
| 商品货号： | TS258946 |
| <strong>Cell line </strong>： | WERI-RB-1 |
| DSMZ no. ： | TS258946 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | retinoblastoma |
| Origin ： | established from retinoblastoma tissue of a 1-year-old Caucasian girl in 1974 |
| Reference(s) ： | 14757, 14758 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing as grape-like clusters of round cells in suspension; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | optimal split ratio of 1:2 to 1:3 once a week; maintain at ca. 0.3-0.5 x 106 cells/ml; maximal cell density of about 1.5 x 106 cells/ml; seed out at ca. 0.3-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 49 hours |
| Harvest ： | cell harvest of about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype with 3.9% polyploidy - 46(41-48)<2n>XX, +6, -10, -10, -14, -22, +3mar, add(3)(q25), add(3)(q25), add(4)(p15), add(5)(q35), i(6q), del(7)(p21), add(9)(q33), der(13)x2, add(16)(q23), add(16)(q23), i(17q), add(19)(q13) - apparently (uniparental?) disomic rearrangement of ch 13 - corresponds to reported karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
