H25B10
H25B10
规格:
货期:
编号:TS258949
品牌:Testobio
| 产品名称： | H25B10 |
|---|---|
| 商品货号： | TS258949 |
| <strong>Cell line </strong>： | H25B10 |
| DSMZ no. ： | TS258949 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti hepatitis B) |
| Origin ： | established by fusion of P3-NSI/1-AG4-1 mouse myeloma cells with spleen cells from a mouse immunized with hepatitis B surface antigen; a monoclonal antibody (IgG1) against hepatitis B surface antigen is produced; patented cell line |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round to oval cells in suspension |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | cells are difficult to culture! maintain at 0.5-1.0 x 105 cells/ml; split 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximal density at about 1-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses ： | ELISA: reverse transcriptase positive |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | H25B10 |
|---|---|
| 商品货号： | TS258949 |
| <strong>Cell line </strong>： | H25B10 |
| DSMZ no. ： | TS258949 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti hepatitis B) |
| Origin ： | established by fusion of P3-NSI/1-AG4-1 mouse myeloma cells with spleen cells from a mouse immunized with hepatitis B surface antigen; a monoclonal antibody (IgG1) against hepatitis B surface antigen is produced; patented cell line |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round to oval cells in suspension |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | cells are difficult to culture! maintain at 0.5-1.0 x 105 cells/ml; split 1:4 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Harvest ： | maximal density at about 1-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses ： | ELISA: reverse transcriptase positive |
